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net目录CONTENTS【背景篇】---01~02/关于我们01~02/四正柏与ELISA的故事03~03/四正柏全国奖励金计划0104【技术篇】---04~04/ELISA技术简介05~06/常见的ELISA类型07~08/ELISA样本制备指南09~10/ELISA实验常见问题解答11~12/品质管理标准13【产品篇】---13~17/人细胞因子ELISA试剂盒18~19/小鼠细胞因子ELISA试剂盒20~20/大鼠细胞因子ELISA试剂盒20~20/猪细胞因子ELISA试剂盒21~21/其他标志物ELISA试剂盒21~21/ELISA辅助试剂【附录】---27~30/SCI文献引用列表272226【服务篇】---22~25/ELISA试剂盒定制服务25~25/ELISA试剂盒检测服务26~26/ELISA试剂盒售前售后服务0201北京四正柏生物科技有限公司(Beijing4ABiotechCo.
,Ltd.
)创办于2009年,地处北京经济技术开发区,是一家集生物医药产品研发、生产、销售、技术服务四位一体的高新技术企业.
四正柏旗下有包括北京东方华辉生物医药在内的多家从属公司,业务涵盖生物医药实验室用品、技术服务、体外诊断试剂(IVD)三大板块.
公司凭借深厚悠久的行业合作背景,与国内外专家合作建成了多个技术平台,产品涉及分子生物学、细胞生物学、免疫学、血液学等领域,多年培育形成了以ELISA、流式细胞术为代表的特色技术优势.
四正柏生物在国内全面建成销售网络,在北上广深等地均设有经营实体,此外还在北美、欧洲等地建成销售渠道,产品销往海外并获市场高度认可.
四正柏生物始终坚持"掌握源头技术,专业创造价值"的发展理念,企业愿景是发展成为国内生命科学和临床诊断领域最具竞争力的高新技术企业,并在国际市场树立强有力的竞争形象.
「1992」年:晶美公司取得当时最大的细胞因子试剂供应商--美国Genzyme公司的独家代理权.
「1993」年:经Genzyme授权和培训,在国内首度分装其DuoSet品牌细胞因子试剂盒.
美方派出技术专家对工艺流程和所有组分的质控(包括包板、干板技术、标本稀释液中基质效应的控制、酶标板选择、试剂瓶防漏测试、标签设计等等)进行了严格的培训和监控,分装产品迅速取得了商业上的成功.
在其后的20年里,国内出现了数十家分装企业,形成了一个独特的区隔市场,而晶美公司一直是这个市场的领导者.
「1998」年:因为竞购流式细胞仪抗体试剂公司PharMingen失利,Genzyme公司放弃科研试剂业务,把细胞因子试剂部门卖给R&DSystem公司.
晶美公司继续代理R&DSystem在中国的销售,直到2008年底.
「2009」年:四正柏生物在原晶美公司研发生产中心的基础上成立,专注于单克隆抗体和细胞因子试剂的开发、生产和相关服务.
经过10年的发展,目前可提供经过国际标准质控的300多种ELISA试剂盒,以极具竞争力的性价比销往全球市场.
27年专注ELISA实验解决方案StorywithELISA四正柏与ELISA的故事Aboutus----ELISAKIT四正柏全国奖励金计划SCHOLARSHIPPROGRAM洗涤除去未结合试剂用捕获抗体包被微孔板封闭洗涤除去未结合试剂加待检样本孵育加生物素化检测抗体孵育加HRP酶-链亲和素结合洗涤弃未结合的检测抗体洗涤除去未结合试剂加终止液并读数加显色底物酶联免疫吸附实验(ELISA)是一种实验室常用的检测方法,广泛应用于测量溶液中的分析物(通常是抗体或抗原)的浓度.
与其他基于抗体的检测方法不同的是,酶联免疫吸附实验(ELISA)或酶免疫测定(ELISA)通过与固相支持物(通常为聚乙烯多孔板)的结合和系列洗涤步骤,实现特异性和非特异性相互作用的分离,并可通过最终有色产物的形成,定量分析原始样品中分析物体的含量.
ELISA实验快速且简单,可迅速分析大量平行的样品,在基础研究和临床诊断实践中广泛使用.
/基本概述Introduction申请方式点击链接http://cn.
mikecrm.
com/3UncvoH或扫描在右方二维码,填写资料并上传PDF全文即可参与.
ELISA实验始于包被,即将抗原或抗体吸附到聚苯乙烯孔板中(吸附通常是溶液被动附着于固体表面上产生一层薄膜),包被后为封闭和检测步骤等(如右简图).
由于ELISA是通过与固相支持物的结合来实现分离分析,因此每个操作步骤间均有重复的洗板操作,以去除未结合的物质.
在此过程中,最关键的就是要充分去除残余溶液,以防止稀释后续步骤加入的试剂.
要获得最佳的一致性结果,可使用自动洗板机来完成洗涤步骤.
ELISA实验虽然原理简单,但仍需谨慎认真对待.
如样品中一些干扰物质的存在,孵育温度的变化等均可影响实验结果.
其中最关键的步骤当属检测环节,因其涉及最终的信号放大以及结果读取.
/基本流程Workow「柏」花点1点=100元奖励金,可兑换现金或产品抵扣券,用于赞助学术会议注册或差旅费.
可以课题组为单位进行累积,有效期为两年.
【IF.
作为民族品牌、高端生物试剂国产化领先者,四正柏通过近十年时间坚持走自主创新之路,建设了数条颇具竞争力的产品线,受到全球科研工作者的青睐.
/奖励条件Requirements/活动概述Introduction/奖励方案RewardProgram/【柏】花点使用方法InstructionsA.
所发表文章涉及实验中有使用四正柏生物自有品牌产品,含:流式抗体、ELISA试剂盒、Annexin-V细胞凋亡检测试剂盒、细胞转染试剂、高敏&超敏ECL化学发光液.
B.
申请奖励时,需提供在线发表的文章链接及PDF全文.
C.
文章发表日期为2019年内,每篇文章仅限申请一次,由第一作者领取和分配.
D.
奖励发放时间,需在正常回款期内.
0304ELISA技术简介IntroductiontoELISAPrinciples常见的ELISA类型TYPESOFELISA直接法主要用于测定抗原间接法主要用于测定抗体0506ELISA可用于测定抗原,也可用于测定抗体.
通常可适用于测定细胞培养上清、血清、血浆以及组织裂解物样品等.
在ELISA检测方法中有三种必要的试剂:①固相的抗原或抗体;②酶标记的抗原或抗体;③酶反应底物.
根据试剂的来源和样品的性质以及检测的实验条件,可设计出各种不同类型的ELISA检测方法.
双抗体夹心法主要用于测定抗原将抗原与固相载体连接,洗涤除去未结合的抗原及杂质.
加酶标抗体进行孵育,固相免疫复合物上的抗原与酶标抗体结合,彻底洗涤未结合的酶标抗体,此时固相上带有的酶量与标本中受检抗原的量相关,加底物反应.
【优点】操作流程简短,实验步骤少;无需使用二抗避免了交叉反应;实验步骤少,操作不易出错;【缺点】实验中的一抗均需直接酶标记,成本相对较高.
/直接法DirectELISA将特异性抗体(通常称为捕获抗体)与固相载体连接,形成固相抗体.
洗涤除去未结合的抗体及杂质.
加入待检样品,恒温孵育.
样品中的特异性抗原与固相抗体结合,形成固相抗原抗体复合物.
洗涤除去其它未结合物质.
加酶标抗体(通常称为检测抗体),保温孵育.
固相免疫复合物上的抗原与酶标抗体结合.
彻底洗涤未结合的酶标抗体.
加底物反应.
高特异性,使用的捕获抗体和检测抗体都是检测物特异性适用于复杂样品,抗原不需进行纯化即可用于检测灵活性更大,灵敏度更高,可采用直接法也可采用间接法.
检测物需要拥有两个以上不同的抗原表位或多个相同的重复表位;不适用于检测小分子物质.
/双抗体夹心法SandwichELISA酶标二抗可加强信号,提高灵敏度灵活性更大,同一酶标二抗可应用于多种不同的一抗不直标一抗,可保留其最多的免疫反应性成本更低,使用的标记抗体更少.
【优点】将抗原与固相载体连结,形成固相抗原.
洗涤除去未结合的抗原及杂质.
加入特异性一抗与固相抗原结合,形成固相抗原抗体复合物.
经洗涤后,加酶标二抗.
固相免疫复合物中的抗体与酶标二抗结合,从而间接地标记上酶.
洗涤后,加底物显色.
【缺点】交叉反应几率升高.
/间接法IndirectELISA竞争法ELISA最为复杂,通常用于检测小分子如半抗原、激素、药物等.
样品中待检游离抗原与固定于固相载体上的抗原一起竞争相同的有限量抗体,当样品中的游离抗原越多,就可结合越多的抗体,而固相抗原则只能结合较少的抗体.
反之亦然.
经洗涤去除样品中抗原与抗体的结合物,只留下固相抗原与抗体的结合物.
显色经计算可得样品中抗原的含量.
竞争法也可根据实验设置直接法,间接法或夹心法形式.
/竞争法CompetitiveELISA无信号表明无带标记的抗体结合到固相载体,样品中抗原的含量很高检测抗体与样品中抗原结合,经洗涤被除去用已知抗原包被固相载体然后封闭加入含未知抗原的样品加入带标记的检测抗体ELISA样本制备指南ELISASAMPLEPREPARATIONGUIDE【细胞培养上清】将细胞培养基移至离心管,并在4℃条件下以1,500rpm离心10min.
立即等分上清液并于-80℃下保存样品.
尽可能减少样品冻融.
【血清】室温血液自然凝固10~20min后,4℃条件下以3,000rpm离心10min.
立即等分上清液并于-80℃下保存样品.
保存过程中如有沉淀形成,应再次离心.
避免反复冻融样品.
【血浆】将全血收集到含抗凝血剂的管中,根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,在4℃条件下以3,000rpm离心10分钟.
立即等分上清液(血浆)并于-80℃下保存样品,避免反复冻融样品.
【唾液】收集样品并在4℃下以10,000xg离心2分钟.
立即等分上清液并在-80℃下保存样品,避免反复冻融样品.
【尿液】用无菌管收集,并在4℃下以10,000xg离心2分钟.
立即等分上清液并于-80℃下保存样品.
避免反复冻融样品.
采集组织(最好在冰上操作,并应尽可能快以防止被蛋白酶降解)样品后,称取组织净重,用液氮迅速冷冻保存备用.
将样品保存在-80℃下以便后续使用或放置在冰上进行直接匀浆.
加入一定量的组织提取缓冲液,用手工或匀浆器将样品匀浆充分.
在4℃下以13000rpm离心20分钟.
置于冰上,将上清液(可溶的蛋白质提取物)等分至新的冷却管中并在-80℃下保存样品.
避免反复冻融样品.
【液体类型样品】液体类实验样品:血清、血浆、尿液、胸腹水、细胞培养上清等【组织样品】08该指南旨在作为教育资源,指南中提供的各种制备方法适用于常规ELISA样品的制备方法通常也会随测定靶标和检测方法而适当变化在建立新的检测方法时,建议查阅相关文献或进行必要的流程优化在收集样品前有必要建立完整的计划,必须清楚要检测的成份是否足够稳定我们提倡新鲜样品尽早检测对收集后当天就进行检测的标本及时储存在4℃备用如有特殊原因需要周期性收集样品在造模取材后,将标本及时分装后放在-20℃或-80℃条件下保存因冰箱与室温存在一定温差,蛋白极易降解,直接影响实验质量,所以避免反复冻融07操作基本流程WorkowELISA实验常见问题解答将洗涤缓冲液加入孔中洗涤,确保孔区域洗涤充分;确保已在洗涤前弃去所有残留抗体溶液;增加洗涤次数;检查偶联物的稀释度,在推荐的稀释度下使用;检查封闭液;延长封闭时间;缩短孵育时间;设置适当对照;新鲜配置缓冲液;重复实验检查试剂配制方法;复核试验试剂添加流程;使用新鲜配制的试剂;检查是否使用了推荐的抗体量;增加抗体用量(浓度);检查使用的试剂;使用ELISA板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;新鲜配置缓冲液;严格按照说明书洗涤流程操作;底物溶液混合后须立即使用;每步均需使用新的封板胶纸或试剂储液槽;新鲜配置缓冲液;检查倍性稀释是否正确;使用酶标板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;检查稀释度是否正确;延长底物溶液孵育时间;严格按照说明书操作流程;重新计算制作新的标准曲线;将洗涤缓冲液加入孔中洗涤,确保孔区域洗涤充分;确保已在洗涤前弃去所有残留溶液;检查封闭步骤是否正确;使用酶标板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;每步均需更换使用新的封板胶纸;须使用封板胶纸;新鲜配置缓冲液;洗板不充分链亲和素-HRP结合物过量孵育时间过长样品或标准品中含干扰物质缓冲液受污染HRP被叠氮化合物污染使用了含胎牛血清的缓冲液复溶抗体缓冲液受污染洗板不充分或跳过洗涤步骤未去除残留的非结合过氧化物酶底物溶液预混太早不新鲜封板胶纸或试剂储液槽重复使用导致HRP残留污染缓冲液有金属或HRP污染链亲和素-HRP结合物量不足检测抗体量不足孵育时间不足实验操作流程有误标准曲线稀释计算错误重复使用封板胶纸未使用封板胶纸缓冲液受污染试剂添加顺序错误或试剂配制不正确封闭不充分抗体用量不足捕获抗体与板结合较差捕获抗体与板结合较差洗板不充分酶标板包被不均匀Part01高背景信号Part02无信号Part05板内重复性差Part03整板呈现规则蓝色Part04标准曲线不佳梯度不明显(值低或曲线平直)0910解决方法可能原因问题解决方法将洗涤缓冲液加入孔中洗涤,确保孔区域洗涤充分;确保已在洗涤前弃去所有残留抗体溶液;严格按照推荐的温度孵育;勿在环境温度变化大的地方孵育;严格按照相同的操作流程;每步均需更换使用新的封板胶纸;新鲜配置缓冲液;设置阳性对照;重复实验;做系列稀释检测回收率;稀释样品重新实验;检查酶标仪;延长显色时间;重新包被新的酶标板;使用酶标板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;检查使用的试剂;孵育时使用封板胶纸;勿在环境温度变化大的地方孵育;确保使用前酶标板处于室温下且试剂也处于室温下;避免使用含这些防腐剂的试剂;Part06板间重复性差Part07标准曲线佳但样品孔无信号Part09整板读数偏低Part10边缘效应Part11显色缓慢问题可能原因洗板不充分操作流程改变封板胶纸重复使用导致HRP残留污染缓冲液受污染样品基质掩盖检测样品中检测物超过标准曲线范围酶标仪波长设置不正确显色时间不充分包被的酶标板超过使用效期使用了含胎牛血清的缓冲液复溶抗体酶标板未处于正确温度下溶液污染孵育温度不均衡捕获抗体与板结合较差孵育温度发生变化样品中无物体检测物或表达量极低Part08标准曲线佳但样品信号偏高ELISATroubleshooting1112四正柏自主品牌产品SCI文献引用(数据截至2019年6月30日)80060040020007302019年6082018年4712017年3542016年2622015年1832014年1162013年682012年282011年62010年四正柏ELISA试剂盒如何确保高品质四正柏ELISA试剂盒的所有生产环节均采用严苛的质量控制标准.
从抗体对的筛选、酶标板的选择、包板、干板、样本稀释液中基质效应的控制、酶底物筛选、试剂瓶防漏测试等环节均经过多重严格的监控,确保提供的每一个试剂盒具有最佳的性能和一致性.
四正柏所有ELISA试剂盒均为完整的、经过验证的即用型试剂盒,可用于定量检测多种复杂样本类型中的靶蛋白含量.
试剂盒采用双抗夹心法免疫检测原理.
甄选全球优质单克隆抗体,利用公司先进的抗体生产和验证平台,进行严苛的抗体对筛选验证.
对多种候选单抗及多抗组合进行差异性测试,严选最佳抗体对组合用于试剂盒开发.
对选择的抗体进行仔细地滴定以确保所选择的浓度可获得最好的检测结果.
在包板过程中,捕获抗体以不同的浓度梯度包被于微孔板,以测试出捕获抗体的最佳结合力和精确度.
检测抗体同样以不同浓度进行测试,以确保最佳浓度下检测抗体与捕获抗体的最佳配对,获得最佳检测信噪比,确保试剂盒高度特异性的检测靶蛋白,具备极高的稳定性.
严格的抗体对筛选标准精密度是试剂盒重复实验时误差大小的评估标准,通常用变异系数CV值表示.
该值是试剂盒的关键性能指标.
A.
可确保多次实验结果的准确性和可重复性;B.
确定两次实验结果是否相同或不同.
变异系数CV值通常需要考虑板内(intra-assay)精密度及板间(inter-assay)精密度.
板内CV值反应同一微孔板上不同板孔间的检测重复性,研究人员可在同一张板上设置复孔测试样品,获得相近结果;板间CV值反应不同批次间不同微孔板的检测重复性,研究人员可在一个较长的时间段使用不同批次试剂盒时获得重复性较好的结果.
四正柏公司所有ELISA试剂盒的板间和板内CV值都控制在10%以内.
精密度与重复性特异性一般指试剂盒出现假阳性和假阴性的概率,也指试剂盒对同类物质的交叉反应率.
抗体交叉反应及基质干扰因素都会影响试剂盒的特异性.
当某种非目标分析物分子与抗体对结合时,发生交叉反应则导致假阳性结果.
当样本基质中某些物质干扰目标分析物与抗体结合时,则发生干扰现象导致假阴性结合.
要防止以上问题的发生,必须经过系列严格的开发和测试过程.
四正柏ELISA试剂盒均会检测与目标检测物同家族的其他蛋白、其他种属相同靶点蛋白以及血清中常见因子的交叉反应率.
同时还会对靶蛋白配体或靶蛋白受体的干扰因素进行测试.
交叉反应及干扰性越小,说明试剂盒的特异性越高.
特异性回收率是试剂盒准确性的判断标准.
复杂样本如血清和血浆等,可能含有干扰因子而影响目标分析物的检测准确性.
添加高、中、低三种浓度的待检目标物标准品到天然样本基质中进行测定,以不添加标准品蛋白的天然样本基质为空白对照,加标的样本所得的结果减去未加标对照样本所得的结果,其差值同加入标准物质的理论值之比即为样品加标回收率.
四正柏ELISA试剂盒的回收率标准是80-120%以内视为合格.
回收率线性是试剂盒准确度和特异性的判断标准.
干扰因子会影响检测的线性,试剂盒需经过严格优化.
四正柏选用高浓度的天然样本或重组蛋白在试剂盒的检测范围内进行梯度稀释,分别测定各浓度下的回收率.
回收率在80-120%之间视为合格.
稀释线性最低检测限是指以恰当的置信概率被检测出的目标分析物的最低浓度值.
一般通过做20个空白对照,最低检测限即该20个空白对照孔OD值的平均值2SD(20个空白对照孔2倍的标准偏差).
最低检测限的值越低说明试剂盒越灵敏.
灵敏度四正柏提供300余种高品质ELISA试剂盒灵敏度高批间差小重复性佳传承27年ELISA开发经验27+YEARSOFELISAMANUFACTURINGEXPERIENCE目录产品表CATALOGUEELISAKITSCXCL16(趋化因子CXCL16)CXCL2/GROβ(人趋化因子CXCL12)CXCL4/PF4(趋化因子CXCL4)CXCL5/ENA-78(趋化因子CXCL5)CXCL6/GCP-2(趋化因子CXCL6)CXCL7/NAP-2(趋化因子CXCL7)DDR1(盘状结构域受体酪氨酸激酶)DPPIV/CD26(二肽酰多肽酶)EGF(表皮生长因子)eNOS(内皮型一氧化氮合酶)FasLigand/TNFSF6(Fas配体)Fas/Apo-1(凋亡相关因子)FetuinA/AHSG(人胎球蛋白A)FGFacidic(酸性成纤维生长因子)FGFbasic(碱性成纤维生长因子)FGF-19(成纤维生长因子19)FGF-21(成纤维生长因子21)FGF-23(成纤维生长因子23)Galectin-3(半乳凝素)G-CSF(粒细胞集落刺激因子)GDF-15(生长分化因子15)GDNF(胶质细胞源性神经营养因子)GM-CSF(粒细胞巨噬细胞集落刺激因子)GranzymeA(人粒酶A)GranzymeB(粒酶B)HB-EGF(肝素结合表皮生长因子)Hepcidin(铁调素)HGF(肝细胞生长因子)HSP60(热休克蛋白60)HSP70/HSPA1A(热休克蛋白70)IFN-α(α干扰素)IFN-β(人β干扰素)IFN-γ(γ干扰素)IgA(免疫球蛋白A)IgE(人免疫球蛋白E)IGFBP-1(胰岛素样生长因子结合蛋白1)IGFBP-7IGFBP-rp1(人胰岛素样生长因子结合蛋白-7)IGF-I(胰岛素样生长因子1)IgGtotal(总免疫球蛋白G)IgM(免疫球蛋白M)IL-1R4/ST2(白细胞介素1受体4)IL-10(白细胞介素10)IL-11(白细胞介素11)7pg/mL7pg/mL7pg/mL0.
5pg/mL7pg/mL7pg/mL78pg/mL7pg/mL15pg/mL40pg/mL15pg/mL70pg/mL15pg/mL60pg/mL30pg/mL7pg/mL15pg/mL40pg/mL31pg/mL4pg/mL4pg/mL15pg/mL7pg/mL15pg/mL20pg/mL4pg/mL4pg/mL60pg/mL300pg/mL60pg/mL7pg/mL4pg/mL7pg/mL0.
8ng/mL234pg/mL15pg/mL39pg/mL39pg/mL15pg/mL0.
8ng/mL7ng/mL15pg/mL7pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells15.
60~1,000pg/mL15.
625~1000pg/mL15.
60~1,000pg/mL1.
95~125pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL156.
00~10000pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL78.
10~5,000pg/mL31.
20~2,000pg/mL156~10,000pg/mL31.
25~2000pg/mL125.
00~8,000pg/mL64~4,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL78.
10~5,000pg/mL62.
50~4,000pg/mL7.
80~500pg/mL7.
8~500pg/mL31.
25~2,000pg/mL15.
60~1,000pg/mL31.
25~2000pg/mL39.
10~2,500pg/mL7.
81~500pg/mL7.
8~500pg/mL125~8,000pg/mL625.
00~40,000pg/mL125.
00~8,000pg/mL15.
60~1,000pg/mL7.
8~500pg/mL15.
60~1,000pg/mL1.
60~100ng/mL468.
75~30000pg/mL31.
20~2,000pg/mL78~5000pg/mL78~5000pg/mL31.
20~2,000pg/mL1.
60~100ng/mL15.
60~1,000ng/mL31.
20~2,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mLCHE0117CHE0278CHE0210CHE0162CHE0214CHE0212CHE0283CHE0131CHE0044CHE0115CHE0196CHE0024CHE0273CHE0202CHE0060CHE0201CHE0199CHE0175CHE0130CHE0025CHE0112CHE0046CHE0026CHE0275CHE0127CHE0144CHE0287CHE0069CHE0192CHE0193CHE0084CHE0274CHE0017CHE0093CHE0270CHE0070CHE0265CHE0265CHE0071CHE0095CHE0096CHE0036CHE0013CHE0010HOTHOTHOTHOTHOTHOTHOT人细胞因子ELISA试剂盒(HumanCytokineELISAKits)检测靶标灵敏度检测范围规格产品编号人细胞因子ELISA试剂盒(HumanCytokineELISAKits)ActivinA(激活素A)ADAMTS5(血小板反应蛋白解整合素金属肽酶5)Adiponectin(脂联素)Amphiregulin(双调蛋白)Angiopoietin-2(血管生成素2)Angiopoietin-like4(血管生成素类似物4)ApolipoproteinA-1/ApoA1(载脂蛋白A1)BAFF/TNFSF13B(B细胞活化因子)BDNF(脑源性神经营养因子)BMP-2(骨形态发生蛋白2)BMP-6(骨形态发生蛋白6)BMP-7(骨形态发生蛋白7)CCL1/I-309(趋化因子CCL1)CCL17/TARC(趋化因子CCL17)CCL19/MIP-3beta(趋化因子CCL19)CCL21(趋化因子CCL21)CCL22/MDC(趋化因子CCL22)CCL24/Eotaxin-2(趋化因子CCL24)CCL25/TECK(趋化因子CCL25)CCL26/Eotaxin-3(趋化因子CCL26)CD27/TNFRSF7(肿瘤坏死因子受体超家族成员7)ComplementComponentC5a(补体成分5a)Corin(丝酪氨酸蛋白酶)CRP(C反应蛋白)Cu/ZnSOD(铜锌超氧化物歧化酶)CXCL1/GRO(趋化因子CXCL1)CXCL10/IP10(趋化因子CXCL10)CXCL11/I-TAC(趋化因子CXCL11)CXCL12/SDF-1(趋化因子CXCL12)CXCL13/BCA-1/BLC(趋化因子CXCL13)CXCL14/BRAK(趋化因子CXCL14)60pg/mL62pg/mL7pg/mL7pg/mL50pg/mL0.
6ng/mL2ng/mL20pg/mL15pg/mL23pg/mL80pg/mL31pg/mL7pg/mL4pg/mL7pg/mL15pg/mL4pg/mL15pg/mL31pg/mL31pg/mL62pg/mL15pg/mL31pg/mL7pg/mL7pg/mL15pg/mL15pg/mL4pg/mL15pg/mL7pg/mL60pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells125~8,000pg/mL125~8000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL93.
80~6,000pg/mL1.
25~80ng/mL3.
12~200ng/mL39.
10~2,500pg/mL32.
50~2,000pg/mL46.
90~3,000pg/mL156.
00~10,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL15.
60~1,000pg/mL31.
25~2000pg/mL7.
81~500pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL62.
50~4,000pg/mL125~8000pg/mL31.
25~2000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL7.
81~500pg/mL31.
25~2,000pg/mL15.
60~1,000pg/mL125.
00~4,000pg/mLCHE0038CHE0292CHE0021CHE0150CHE0153CHE0154CHE0246CHE0076CHE0041CHE0078CHE0184CHE0182CHE0226CHE0220CHE0218CHE0140CHE0219CHE0224CHE0229CHE0222CHE0285CHE0296CHE0172CHE0104CHE0088CHE0065CHE0091CHE0213CHE0068CHE0211CHE0215检测靶标灵敏度检测范围规格产品编号HOT1314HOTIL-12p40(白细胞介素12p40)IL-12p70(白细胞介素12p70)IL-13(白细胞介素13)IL-15(白细胞介素15)IL-17(白细胞介素17)IL-17C(人白介素17C)IL-17E(人白介素17E)IL-18(白细胞介素18)IL-18BPaElisaKit(人IL-18结合蛋白alpha)IL-19(白细胞介素19)IL-1(白细胞介素1)IL-1α(白细胞介素1α)IL-2(白细胞介素2)IL-21(白细胞介素21)IL-22(白细胞介素22)IL-23(白细胞介素23)IL-24/MDA-7(白细胞介素24)IL-27(白细胞介素27)IL-28/IFN-lambda2(白细胞介素28)IL-29/IFNlambda1(白细胞介素29)IL-3(白细胞介素3)IL-31(白细胞介素31)IL-32α(白细胞介素32α)IL-33(白细胞介素33)IL-34(白细胞介素34)IL-36gamma/IL-1F9(白细胞介素36gamma)IL-37/IL-1F7(白细胞介素37)IL-4(白细胞介素4)IL-5(白细胞介素5)IL-6(白细胞介素6)IL-6Ralpha(白细胞介素6受体α)IL-6sRα(可溶性白细胞介素6受体α)IL-7(白细胞介素7)IL-8(白细胞介素8)IL-9(白细胞介素9)Irisin/FNDC5(鸢尾素)KGF/FGF-7(角化细胞生长因子)Ki-67/MKI67(增殖标记物Ki-67)Klotho(klotho跨膜蛋白)Leptin(瘦素)LIF(白血病抑制因子)lipocalin-2/NGAL(载脂蛋白2)MCP-1(单核细胞趋化蛋白1)MCP-2/CCL8(单核细胞趋化蛋白2)30pg/mL15pg/mL1pg/mL4pg/mL7pg/mL60pg/mL6pg/mL20pg/mL47pg/mL15pg/mL4pg/mL2pg/mL4pg/mL16pg/mL16pg/mL16pg/mL31pg/mL31pg/mL60pg/mL7pg/mL15pg/mL60pg/mL4pg/mL16pg/mL30pg/mL15pg/mL15pg/mL4pg/mL1pg/mL2pg/mL7pg/mL7pg/mL4pg/mL7pg/mL2pg/mL62pg/mL15pg/mL3pg/mL40pg/mL15pg/mL7pg/mL40pg/mL7pg/mL0.
8pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells62.
50~4,000pg/mL31.
25~2,000pg/mL3.
12~200pg/mL7.
81~500pg/mL15.
6~1,000pg/mL125.
00~8,000pg/mL11.
70~750pg/mL78~5,000pg/mL93.
75~6000pg/mL31.
25~2,000pg/mL7.
81~500pg/mL3.
91~250pg/mL7.
81~500pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL62.
50~4,000pg/mL64~8,000pg/mL125~8,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL125~8,000pg/mL7.
81~500pg/mL32.
25~2,000pg/mL64~4,000pg/mL31.
25~2000pg/mL31.
20~2,000pg/mL7.
81~500pg/mL1.
60~100pg/mL3.
90~250pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL15.
60~1,000pg/mL3.
90~250pg/mL125~8000pg/mL31.
20~2,000pg/mL6.
25~400pg/mL39~2500pg/ml31.
25~2,000pg/mL15.
60~1,000pg/mL78~5,000pg/mL15.
60~1,000pg/mL1.
56~100pg/mLCHE0016CHE0015CHE0004CHE0055CHE0054CHE0251CHE0252CHE0007CHE0289CHE0018CHE0001CHE0101CHE0003CHE0056CHE0057CHE0008CHE0085CHE0022CHE0030CHE0031CHE0028CHE0032CHE0058CHE0034CHE0037CHE0301CHE0174CHE0005CHE0006CHE0009CHE0204CHE0261CHE0143CHE0011CHE0012CHE0303CHE0232CHE0198CHE0129CHE0053CHE0083CHE0121CHE0103CHE0062人细胞因子ELISA试剂盒(HumanCytokineELISAKits)检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOTHOTHOTHOTHOTHOTHOTHOTHOT人细胞因子ELISA试剂盒(HumanCytokineELISAKits)HOTMCP-3(单核细胞趋化蛋白3)M-CSF(巨噬细胞集落刺激因子)MIF(巨噬细胞移动抑制因子)MIG/CXCL9(γ干扰素诱导单核细胞因子)MIP-3α/CCL20(巨噬细胞炎性蛋白3α)MMP-13(基质金属蛋白酶13)MMP-2(基质金属蛋白酶2)MMP-3(基质金属蛋白酶3)MMP-9(基质金属蛋白酶9)MyD88(髓分化因子88)Myeloperoxidase(髓过氧化物酶)β-NGF(β-神经生长因子)OPG(护骨素)OSM-R(抑瘤素M受体)OsteepontinOPN(骨桥蛋白)Park7/DJ-1(帕金森氏病蛋白7)PBEF/Visfatin(前B细胞克隆增强因子)PD-1(程序性细胞死亡因子受体1)PDGF-AA(血小板衍化生长因子AA)PDGF-AB(血小板衍化生长因子AB)PDGF-BB(血小板衍化生长因子BB)PDGF-DD(血小板衍化生长因子DD)Periostin/OSF-2(成骨细胞特异性因子2)Peroxiredoxin2(人过氧化物酶2)PIGF(胎盘生长因子)Pro-CollagenIalpha1(I型胶原α1前体)Proinsulin(胰岛素原)Prolactin(催乳素)P-selectin(P选择素)Rantes(调节活化的正常T细胞表达和分泌因子)RBP4(视黄醇结合蛋白4)Resistin(抵抗素)S100B(S100B蛋白)sCD40L(可溶性CD40配体)SerpinE1/PAI-1(纤溶酶原激活物抑制剂1)sE-selectin(可溶性E-选择素)sFRP-5(分泌性卷曲相关蛋白)sICAM-1(可溶性细胞间粘附分子1)sIL-2R(白细胞介素2可溶性受体)sTNFRI/TNFRSF1A(可溶性肿瘤坏死因子受体)sTNFRII/TNFRSF1B(可溶性肿瘤坏死因子受体)Syndecan-1(多配体蛋白聚糖1)TGF-α(转化生长因子α)TGF-β1(转化生长因子β1)4pg/mL7pg/mL15pg/mL31pg/mL7pg/mL31pg/mL0.
31pg/mL15pg/mL30pg/mL78pg/mL31pg/mL15pg/mL15pg/mL80pg/mL30pg/mL32pg/mL2ng/mL80pg/mL7pg/mL7pg/mL15pg/mL4pg/mL30pg/mL390pg/mL15pg/mL7pg/mL70pg/mL15pg/mL30pg/mL7pg/mL12pg/mL4pg/mL25pg/mL40pg/mL0.
15ng/mL40pg/mL15pg/mL30pg/mL15pg/mL8pg/mL4pg/mL62pg/mL4pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells7.
81~500pg/mL15.
60~1,000pg/mL32~2,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL0.
63~20ng/mL31.
20~2,000pg/mL62.
50~4,000pg/mL156.
25~10000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL62.
50~4,000pg/mL312~20,000pg/mL64~4,000pg/mL62.
50~4000pg/mL3.
125~200ng/mL156.
00~10,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL7.
81~500pg/mL62.
50~4,000pg/mL781.
25~50000pg/mL31.
20~2,000pg/mL31.
25~2,000pg/mL156~10,000pg/mL31.
25~2,000pg/mL125~8,000pg/mL15.
60~1,000pg/mL23.
44~1500pg/mL7.
81~500pg/mL46.
90~3,000pg/mL156~10,000pg/mL0.
312~20ng/mL78~5,000pg/mL31.
25~2000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL12.
50~800pg/mL7.
81~500pg/mL125~8000pg/mL7.
8~500pg/mL31.
25~2,000pg/mLCHE0206CHE0042CHE0118CHE0067CHE0061CHE0179CHE0098CHE0177CHE0105CHE0294CHE0113CHE0047CHE0074CHE0087CHE0122CHE0282CHE0086CHE0209CHE0238CHE0072CHE0073CHE0239CHE0075CHE0277CHE0082CHE0114CHE0033CHE0281CHE0106CHE0092CHE0286CHE0035CHE0244CHE0109CHE0120CHE0108CHE0295CHE0052CHE0027CHE0170CHE0158CHE0300CHE0116CHE0029检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOT1516人细胞因子ELISA试剂盒(HumanCytokineELISAKits)TGF-β3(转化生长因子β3)Thrombomodulin/BDCA-3(凝血酶调节蛋白)Thrombopoietin(促血小板生成素)Thrombospondin-1(人血小板反应蛋白-1)TIM-1/KIM-1(T细胞免疫球蛋白黏蛋白)TIMP-1(基质金属蛋白酶抑制因子1)TIMP-2(基质金属蛋白酶抑制因子2)TL1A/TNFSFS15(肿瘤坏死因子超家族15)TLR2(Toll样受体2)TNF-α(肿瘤坏死因子α)TNF-β(肿瘤坏死因子β)TotalMMP-1(总基质金属蛋白酶1)TRAIL/TNFSF10(肿瘤坏死因子相关凋亡诱导配体)TRANCE/RANKL(肿瘤坏死因子相关激活诱导因子)TSLP(胸腺基质淋巴细胞生成素)Uromodulin(尿调节素)VCAM-1(血管内皮细胞粘附因子1)VEGF(血管内皮生长因子)15pg/mL15pg/mL45pg/mL0.
78ng/mL7pg/mL30pg/mL15pg/mL16pg/mL60pg/mL4pg/mL15pg/mL80pg/mL12pg/mL39pg/mL15pg/mL31pg/mL7pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells31.
20~2,000pg/mL31.
20~2,000pg/mL93~6,000pg/mL1.
563~100ng/mL15.
60~1,000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL31.
25~2000pg/mL125.
00~8,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL156.
00~10,000pg/mL23.
44~1500pg/mL78~5000pg/mL31.
20~2,000pg/mL62.
5~4000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mLCHE0171CHE0149CHE0124CHE0271CHE0132CHE0107CHE0100CHE0288CHE0188CHE0019CHE0260CHE0097CHE0293CHE0297CHE0059CHE0302CHE0110CHE0043检测靶标灵敏度检测范围规格产品编号Adiponectin/Acrp3(脂联素)alpha-Fetoprotein/AFP(甲胎蛋白)BAFF/BLyS/TNFSF13B(B细胞活化因子)BDNF(脑源性神经营养因子)CCL11/Eotaxin-1(趋化因子CCL11)CCL24/Eotaxin/MPIF-2(趋化因子CCL24)CCL5/Rantes(趋化因子CCL5)Chemerin(脂肪因子)ComplementComponentC5a(补体C5a)CRP(C反应蛋白)CX3CL1/Fractalkine(趋化因子cx3cl1)CXCL10/IP10(趋化因子CXCL10)CXCL11/I-TAC(趋化因子CXCL11)CXCL12/SDF-1(趋化因子CXCL12)CXCL15/Lungkine(趋化因子CXCL15)CXCL7/ThymusChemokine-1(趋化因子CXCL7)CXCL9/MIG(趋化因子CXCL9)EGF(表皮生长因子)Erythropoietin(促红细胞生成素)E-Selectin/CD62E(E-选择素)FGFbasic(碱性成纤维生长因子)FGF-21(成纤维生长因子21)G-CSF(粒细胞集落刺激因子)GDF-15(生长分化因子15)GM-CSF(粒细胞巨噬细胞集落刺激因子)GranzymeB(粒酶B)HGF(肝细胞生长因子)IFN-beta(小鼠β干扰素)IFN-γ(γ干扰素)IgA(免疫球蛋白A)IgE(免疫球蛋白E)IGF-I(胰岛素样生长因子-1)IgGtotal(总免疫球蛋白G)IgM(免疫球蛋白M)IL-10(白细胞介素10)IL-12/23(P40)(白细胞介素12)IL-12p70(白细胞介素12p70)IL-13(白细胞介素13)IL-15(白细胞介素15)IL-17A(白细胞介素17A)IL-17F(白细胞介素17F)IL-1ra/IL-1F3(白细胞介素1受体a)IL-1(白细胞介素1)IL-1α(白细胞介素1α)15pg/mL31pg/mL30pg/mL7pg/mL4pg/mL7pg/mL15pg/mL8pg/mL7pmol/l15pg/mL0.
2ng/mL30pg/mL31pg/mL25pg/mL60pg/mL4pg/mL7pg/mL2pg/mL32pg/mL31pg/mL30pg/mL7pg/mL8pg/mL15pg/mL16pg/mL20pg/mL80pg/mL6pg/mL4pg/mL0.
20ng/mL156pg/mL15pg/mL0.
8ng/mL0.
20ng/mL7pg/mL4pg/mL7pg/mL4pg/mL60pg/mL4pg/mL15pg/mL80pg/mL7pg/mL1pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells31.
20~2,000pg/mL62.
50~4,000pg/mL62.
50~4000pg/mL15.
625~1000pg/mL7.
81~500pg/mL15.
60~1,000pg/mL31.
20~2000pg/mL15.
625~1000pg/mL15.
60~1000pmol/l23.
40~1,500pg/mL0.
39~25ng/mL62.
50~4000pg/mL62.
50~4,000pg/mL46.
90~3,000pg/mL125.
00~8,000pg/mL7.
81~500pg/mL15.
60~1000pg/mL3.
91~250pg/mL62.
5~4000pg/mL62.
50~4,000pg/mL64~4000pg/mL15.
60~1000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL40~5000pg/mL156~10000pg/mL12.
5~800pg/mL7.
8~500pg/mL0.
39~25ng/mL312~20,000pg/mL31.
20~2000pg/mL1.
56~100ng/mL0.
39~25ng/mL15.
6~1000pg/mL7.
8~500pg/mL15.
6~1000pg/mL7.
8~500pg/mL125~8000pg/mL7.
8~500pg/mL23.
40~1,500pg/mL156.
00~10,000pg/mL31.
25~2000pg/mL1.
8~125pg/mLCME0103CME0110CME0055CME0120CME0079CME0070CME0048CME0119CME0066CME0104CME0125CME0051CME0073CME0065CME0076CME0075CME0050CME0038CME0126CME0095CME0039CME0040CME0030CME0042CME0026CME0057CME0052CME0116CME0003CME0058CME0029CME0053CME0059CME0060CME0016CME0024CME0013CME0009CME0010CME0041CME0089CME0090CME0015CME0019v检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOT小鼠细胞因子ELISA试剂盒(MouseCytokineELISAKits)HOTHOTHOTHOTHOTHOTHOT17184pg/mL16pg/mL2pg/mL4pg/mL16pg/mL16pg/mL16pg/mL7pg/mL7pg/mL2pg/mL15pg/mL7pg/mL7pg/mL60pg/mL7pg/mL30pg/mL7pg/mL0.
8pg/mL7pg/mL7pg/mL60pg/mL40pg/mL62.
5pg/mL6pg/mL4pg/mL60pg/mL7pg/mL156pg/mL60pg/mL15pg/mL50pg/mL32pg/mL7pg/mL4pg/mL15pg/mL4pg/mL60pg/mL4pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells15.
6~1000pg/mL31.
25~2000pg/mL3.
9~250pg/mL15.
6~1000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL15.
6~1000pg/mL7.
8~500pg/mL31.
20~2,000pg/mL15.
6~1000pg/mL15.
625~1000pg/mL125~8000pg/mL15.
625~1000pg/mL62.
5~4000pg/mL15.
60~1,000pg/mL1.
56~100pg/mL15.
60~1,000pg/mL15.
6~1000pg/mL125.
00~8,000pg/mL78.
10~5,000pg/mL125~16,000pg/mL12.
5~800pg/mL7.
8~500pg/mL125.
00~8,000pg/mL15.
60~1000pg/mL312.
5~20000pg/mL125~8000pg/mL31.
25~2000pg/mL93.
80~6,000pg/mL62.
5~4000pg/mL15.
625~1000pg/mL7.
8~500pg/mL62.
50~4000pg/mL7.
8~500pg/mL125~8000pg/mL15.
6~1000pg/mLCME0001CME0021CME0033CME0028CME0025CME0035CME0036CME0005CME0023CME0006CME0031CME0008CME0002CME0061CME0123CME0046CME0084CME0047CME0078CME0018CME0098CME0099CME0115CME0121CME0062CME0105CME0045CME0124CME0064CME0020CME0032CME0127CME0122CME0004CME0054CME0044CME0043CME0014检测靶标灵敏度检测范围规格产品编号CRP(C反应蛋白)EGF(表皮生长因子)GM-CSF(粒细胞巨噬细胞集落刺激因子)ICAM-1/CD54(细胞间黏连分子1)IFN-γ(γ干扰素)IGF-I(胰岛素样生长因子)IgGtotal(总免疫球蛋白G)IgM(免疫球蛋白M)IL-10(白细胞介素10)IL-17A(白细胞介素17A)IL-1(白细胞介素1)IL-1α(白细胞介素1α)IL-2(白细胞介素2)IL-4(白细胞介素4)IL-6(白细胞介素6)MCP-1(单核细胞趋化蛋白-1)MIP-2(巨噬细胞炎性蛋白2)NGAL/Lipocalin-2(中性粒细胞明胶酶相关脂质运载蛋白)TGF-β1(转化生长因子β1)β-NGF(大鼠神经生长因子-β)TIMP-1(金属蛋白酶1组织抑制剂)TNF-α(肿瘤坏死因子α)TotalMMP-9(总基质金属蛋白酶9)VEGF(血管内皮生长因子)80pg/mL7pg/mL7pg/mL31pg/mL15pg/mL16pg/mL0.
8ng/mL0.
16ng/mL15pg/mL7pg/mL30pg/mL7pg/mL7pg/mL7pg/mL60pg/mL7pg/mL7pg/mL40pg/mL31pg/mL7pg/mL15pg/mL7pg/mL40pg/mL7pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells156.
00~10,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL31.
25~2000pg/mL1.
60~100ng/mL0.
312~20ng/mL31.
25~2,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL15.
60~1,000pg/mL125~8,000pg/mL15.
625~1000pg/mL15.
60~1,000pg/mL78.
10~5,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL31.
25~2,000pg/mL78.
10~5,000pg/mL15.
60~1,000pg/mLCRE0025CRE0030CRE0020CRE0031CRE0002CRE0033CRE0016CRE0017CRE0007CRE0021CRE0006CRE0015CRE0001CRE0004CRE0005CRE0034CRE0009CRE0018CRE0012CRE0019CRE0029CRE0003CRE0026CRE0010检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOTHOTHOTHOT小鼠细胞因子ELISA试剂盒(MouseCytokineELISAKits)HOTHOTIL-2(白细胞介素2)IL-21(白细胞介素21)IL-22(白细胞介素22)IL-23(白细胞介素23)IL-25/IL-17E(白细胞介素25/17E)IL-27(白细胞介素27)IL-33(白细胞介素33)IL-4(白细胞介素4)IL-5(白细胞介素5)IL-6(白细胞介素6)IL-7(白细胞介素7)IL-8/CXCL1/CXCL1(白细胞介素8)IL-9(白细胞介素9)Leptin(瘦素)LIX(脂多糖诱导的cxc趋化因子)MCP-1(单核细胞趋化蛋白1)MCP-2/CCL8(单核细胞趋化蛋白2)MIP-1alpha/CCL3(巨噬细胞炎性蛋白1α)MIP-1beta/CCL4(巨噬细胞炎性蛋白1β)MIP-2/CXCL2(巨噬细胞炎性蛋白2)MMP-3(基质金属蛋白酶3)MMP-9(基质金属蛋白酶9)Myeloperxidase(髓过氧化物酶)NeutrophilElastase/ELA2(中性白细胞弹性蛋白酶)NGAL/Lipocalin-2(中性粒细胞明胶酶相关脂质运载蛋白)PD-1(程序性细胞死亡因子受体1)PlGF-2(胎盘生长因子2)Prolactin(催乳素)sICAM-1(可溶性细胞间粘附分子1)TGF-β1(转化生长因子β1)Thrombomodulin/BDCA-3(凝血酶调节蛋白)Thrombopoietin(促血小板生成素)TL1A/TNFSF15(肿瘤坏死因子超家族15)TNF-α(肿瘤坏死因子α)TRANCE/RANKL(核因子κB受体活化因子配体)TSLP(胸腺基质淋巴细胞生成素)VCAM-1(血管内皮细胞粘附因子1)VEGF(血管内皮生长因子)大鼠细胞因子ELISA试剂盒(RatCytokineELISAKits)HOTHOTHOTHOTHOTHOT7pg/mL7pg/mL40pg/mL30pg/mL7pg/mL15pg/mL60pg/mL30pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells15.
60~1,000pg/mL15.
60~1,000pg/mL78~5,000pg/mL62.
50~4,000pg/mL23.
44~1,500pg/mL31.
25~2,000pg/mL125~8,000pg/mL62.
50~4,000pg/mL31.
25~2000pg/mLCSE0004CSE0003CSE0012CSE0013CSE0001CSE0002CSE0006CSE0008CSE0005检测靶标灵敏度检测范围规格产品编号IFN-γ(γ干扰素)IL-10(白细胞介素10)IL-12/IL-23p40(白细胞介素12/白细胞介素23p40)IL-1β(白细胞介素1β)IL-2(白细胞介素2)IL-4(白细胞介素4)IL-6(白细胞介素6)IL-8(白细胞介素8)TNF-α(肿瘤坏死因子α)猪细胞因子ELISA试剂盒(SwineCytokineELISAKits)1920Glucagon(高血糖素)Insulin(胰岛素)PDGF-BB(血小板衍化生长因子-BB)TotalHIF-1alpha(总低氧诱导因子1α)TotalHSP70/HSPA1A(总热休克蛋白70)7pg/mL7pmol/l31pg/mL60pg/mL80pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells31.
25~2,000pg/mL15.
60~1,000pmol/l62.
50~4,000pg/mL125~8,000pg/mL156~10,000pg/mLCSPE0012CSPE0011CSPE0010CSPE0001CSPE0002检测靶标灵敏度检测范围规格产品编号其他标志物ELISA试剂盒(OtherCytokineELISAKits)SFQ012SFQ007SFQ028SFQ011SFQ027SFQ0103SFQ008SFQ005SFQ0102SFQ001SFQ0101SFQ009包被液标准品封板膜封闭液高吸附酶标板酶结合物稀释液浓缩洗涤液(20X)生物素化抗体生物素化抗体稀释液显色剂/单组份超灵敏TMB样本稀释液终止液100ml支张10ml块16ml25ml支/48T16ml6ml16ml12ml产品编号产品名称产品规格ELISA辅助试剂(ELISAAccessoryReagents)定制化服务产品表ADAMTS13(血管性血友病因子裂解蛋白酶)A-FABP(脂肪型脂肪酸结合蛋白)alpha-Defensin1(防御素α1)alpha-Fetoprotein(甲胎蛋白)Angiopoietin-1(血管生成素1)BCMA/TNFRSF17(B细胞成熟蛋白)BMP-10(骨形态发生蛋白10)BMP-11(骨形态发生蛋白11)BMP-4(骨形态发生蛋白4)BMP-5(骨形态发生蛋白5)BMP-9(骨形态发生蛋白9)CCL11/Eotaxin(趋化因子CCL11)CCL14a/HCC-1(趋化因子CCL14a)CCL15/MIP-1delta/LKN-1(趋化因子CCL15)CCL16/HCC-4(趋化因子CCL16)CCL18/PARC(趋化因子CCL18)CCL23/MPIF-1(趋化因子CCL23)CCL27/CTACK(趋化因子CCL27)CCL28(趋化因子CCL28)CNTF(睫状神经营养因子)ComplementFactorD(补体因子D)ComplementFactorH(补体因子H)CX3CL1/Fractalkine(趋化因子CX3CL1)CytochromeC(细胞色素C)E-Cadherin(E-钙黏蛋白)Endoglin/CD105(内皮因子)Endostain(内皮抑素)ErbB2/Her2(总酪氨酸激酶受体)FABP2(脂肪酸结合蛋白2)FGF-4(成纤维生长因子-4)FGF-9(成纤维生长因子9)FGF-BP(成纤维生长因子结合蛋白)HumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHuman96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells0.
78~50ng/mL62.
50~4,000pg/mL0.
50~32ng/mL78.
10~5,000pg/mL156~10,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL7.
80~500pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL31.
20~2,000pg/mL0.
206~150ng/mL0.
63~20ng/mL31.
25~2,000pg/mL187.
00~12,000pg/mL125~8,000pg/mL64~4,000pg/mL62.
50~4,000pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL62.
50~4,000pg/mL23.
40~1,500pg/mLCHE0156CHE0245CHE0050CHE0249CHE0168CHE0102CHE0187CHE0186CHE0183CHE0185CHE0079CHE0217CHE0230CHE0223CHE0231CHE0133CHE0228CHE0225CHE0141CHE0040CHE0207CHE0208CHE0139CHE0023CHE0195CHE0138CHE0119CHE0194CHE0111CHE0049CHE0200CHE0235检测靶标反应种属0.
4ng/mL31pg/mL0.
25ng/mL40pg/mL80pg/mL15pg/mL7pg/mL15pg/mL7pg/mL31pg/mL8pg/mL7pg/mL7pg/mL7pg/mL4pg/mL4pg/mL7pg/mL31pg/mL7pg/mL15pg/mL15pg/mL0.
1ng/mL0.
3ng/mL15pg/mL100pg/mL60pg/mL30pg/mL31pg/mL15pg/mL30pg/mL31pg/mL10pg/mL灵敏度检测范围规格产品编号ELISA试剂盒定制服务四正柏生物秉承以客户为中心的原则,最大程度满足客户实验需求,目前推出ELISA试剂盒定制服务,客户提出需检测的特定靶标,四正柏将为您定制您专属的ELISA试剂盒.
目前,以下列表中的靶标均可接受定制服务.
更多定制靶标,请联系四正柏生物.
定制说明:【最低起订量】96wells/【定制周期】5~6周CUSTOMELISAKITS2122HO-1/HMOX1(血红素加氧酶1)HSP27(热休克蛋白27)IgD(免疫球蛋白D)IL-13Ralpha2(白细胞介素13受体α2)IL-16(白细胞介素16)IL-1ra/IL-1F3(白细胞介素1受体α)IL-20(白细胞介素20)IL-36Ra/IL-1F5(白细胞介素36受体a)LeptinR(瘦素受体)L-Selectin/CD62L(L选择素)MCP-4/CCL13(单核细胞趋化蛋白4)Mesothelin(间皮素)MICA(跨膜糖蛋白)MICB(跨膜糖蛋白)MIP-1α/CCL3(巨噬细胞炎性蛋白1α)MIP-1β/CCL4(巨噬细胞炎性蛋白1β)MIP-3β/CCL19(巨噬细胞炎性蛋白3β)MMP-10(基质金属蛋白酶10)MMP-7(基质金属蛋白酶7)NGFR(神经生长因子受体)Notch1(Notch1蛋白)NRG1-β1(神经调节蛋白)NT-4(神经营养因子4)Oncostatin(抑癌素)proBDNF(脑源性神经生长因子前体)Pro-MMP-1(基质金属蛋白酶1前体)Pro-MMP-13(基质金属蛋白酶13前体)SCF(干细胞因子)SerumAlbumin(血清白蛋白)TLR3(Toll样受体3)TNFRI/TNFRSF1A(肿瘤坏死因子受体1)TotalFGFR2alpha(总成纤维细胞生长因子受体2)TotalFGFR3(总成纤维细胞生长因子受体3)TotalFGFR4(总成纤维细胞生长因子受体4)TotalMMP-8(总基质金属蛋白酶8)TotalSurvivin(生存素)Trappin-2(细胞蛋白酶抑制因子)TWEAK/TNFSF12(肿瘤坏死因子相关弱凋亡诱导因子)Ubiquitin+1(泛素+1)u-PlasminogenActivator/Urokinase(纤溶酶原)VEGFR1/Flt-1(血管内皮生长因子受体1)βcatenin(β-连环蛋白)IGFBP-3(胰岛素样生长因子结合蛋白3)Granulysin(颗粒溶素)80pg/mL15pg/mL4ng/mL0.
2ng/mL7pg/mL20pg/mL30pg/mL50pg/mL0.
15ng/mL40pg/mL4pg/mL35pg/mL31pg/mL70pg/mL4pg/mL7pg/mL7pg/mL15pg/mL15pg/mL31pg/mL40pg/mL60pg/mL15pg/mL15pg/mL40pg/mL30pg/mL31pg/mL15pg/mL1.
5ng/mL50pg/mL7pg/mL80pg/mL130pg/mL80pg/mL31pg/mL31pg/mL15pg/mL30pg/mL60pg/mL30pg/mL70pg/mL150pg/mL62pg/mL7pg/mL96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells156.
00~10,000pg/mL31.
20~2,000pg/mL7.
80~1,000ng/mL0.
312~20ng/mL15.
6~1,000pg/mL39.
10~2,500pg/mL62.
50~4,000pg/mL93.
80~6,000pg/mL0.
312~20ng/mL78.
10~5,000pg/mL7.
81~500pg/mL78~5,000pg/mL62.
50~4,000pg/mL156.
00~10,000pg/mL7.
80~500pg/mL15.
60~1,000pg/mL15.
60~1000pg/mL31.
20~2,000pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL78.
10~5,000pg/mL125~4,000pg/mL31.
20~2,000pg/mL31.
20~2,000pg/mL78.
10~5,000pg/mL46.
90~3,000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL2.
50~160ng/mL93.
80~6,000pg/mL12.
50~800pg/mL156.
00~10,000pg/mL250.
00~16,000pg/mL156.
00~10,000pg/mL62.
50~4,000pg/mL62.
50~4,000pg/mL31.
20~2000pg/mL62.
50~4,000pg/mL125.
00~8,000pg/mL64~4,001pg/mL156.
00~10,000pg/mL312.
00~20,000pg/mL125~8000pg/mL15.
6~1000pg/mLCHE0190CHE0191CHE0094CHE0205CHE0014CHE0142CHE0020CHE0173CHE0237CHE0243CHE0221CHE0126CHE0146CHE0147CHE0051CHE0125CHE0203CHE0180CHE0178CHE0163CHE0247CHE0164CHE0039CHE0169CHE0137CHE0197CHE0181CHE0080CHE0157CHE0176CHE0159CHE0236CHE0234CHE0233CHE0099CHE0165CHE0167CHE0077CHE0248CHE0123CHE0152CHE0241CHE0280CHE0284检测靶标灵敏度HumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHuman反应种属检测范围规格产品编号2324定制化服务产品表定制化服务产品表COMP(软骨寡聚基质蛋白)Pentraxin3/TSG-14(穿透素3/肿瘤坏死因子诱导基因14)LysylOxidaseHomolog2(赖氨酰氧化酶样蛋白2)ADAMTS4(金属肽酶含血小板反应蛋白基元4)BMP-9(骨形态发生蛋白9)CCL1/TCA-3(趋化因子CCL1)CCL17/TARC(趋化因子CCL17)CCL21/6Ckine(趋化因子CCL21)CCL22/MDC(趋化因子CCL22)CCL25/TECK(趋化因子CCL25)CCL27/CTACK(趋化因子CCL27)CCL28(趋化因子CCL28)CCL6/C10(趋化因子CCL6)CD40Ligand/TNFSF5(CD40配体)CXCL13/BLC/BCA-1(趋化因子CXCL13)CXCL16(趋化因子CXCL16)CXCL4/PF4(趋化因子CXCL4)FasLigand/TNFSF6(Fas配体)Fas/TNFRSF6/CD95(凋亡调节表面抗原Fas)FGFacidic(酸性成纤维生长因子)Gremlin(细胞因子Gremlin)IFN-zeta/Limitin(ζ干扰素)IL-11(白细胞介素11)IL-15/IL-15RComplex(白细胞介素15及其受体复合物)IL-17A/FHeterodimer(白细胞介素17A/F二聚体)IL-19(白细胞介素19)IL-20(白细胞介素20)IL-24(白细胞介素24)IL-3(白细胞介素3)IL-38(白细胞介素38)LeptinR(瘦素受体)L-Selectin/CD62L(L-选择素)MCP-5/CCL12(单核细胞趋化蛋白5)M-CSF(巨噬细胞集落刺激因子)MIP-1gamma/CCL9(巨噬细胞炎性蛋白1γ)MIP-3alpha/CCL20(巨噬细胞炎性蛋白3α)MIP-3beta/CCL19(巨噬细胞炎性蛋白3β)Pro-MMP-9(基质金属蛋白酶9前体)P-Selectin/CD62P(P-选择素)Resistin(抵抗素)SCF(干细胞因子)VitaminDBP(维生素D结合蛋白)IFN-α(干扰素-α)LIF(白血病抑制因子)20pg/mL110pg/mL0.
2ng/Ml7pg/mL7pg/mL4pg/mL15pg/mL7pg/mL4pg/mL15pg/mL31pg/mL7pg/mL7pg/mL50pg/mL7pg/mL7pg/mL15pg/mL7pg/mL31pg/mL7pg/mL40pg/mL7pg/mL60pg/mL4pg/mL25pg/mL30pg/mL16pg/mL4pg/mL4pg/mL125pg/mL15pg/mL60pg/mL15pg/mL7pg/mL4pg/mL15pg/mL7pg/mL0.
20ng/mL31pg/mL7pg/mL31pg/mL250pg/mL7pg/mL3.
15pg/mL96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells39.
1~2500pg/mL218.
0~14000pg/mL0.
4~24ng/mL15.
6~1000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL7.
8~500pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL15.
6~1000pg/mL15.
60~1,000pg/mL93.
80~6,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL12.
50~800pg/mL93.
80~6,000pg/mL15.
60~1,000pg/mL125~8000pg/mL7.
8~500pg/mL46.
90~3,000pg/mL62.
5~4,000pg/mL31.
20~2000pg/mL7.
8~500pg/mL7.
81~500pg/mL250~16000pg/mL31.
20~2,000pg/mL125.
00~8,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL0.
312~20ng/mL62.
50~4,000pg/mL15.
60~1,000pg/mL62.
50~4000pg/mL469.
00~15,000pg/mL15.
60~1,000pg/mL6.
30~400pg/mLCHE0291CHE0298CHE0299CHE0304CME0111CME0083CME0068CME0118CME0063CME0087CME0086CME0049CME0088CME0102CME0072CME0074CME0071CME0092CME0093CME0097CME0109CME0106CME0007CME0011CME0091CME0012CME0017CME0034CME0027CME0037CME0107CME0096CME0085CME0108CME0082CME0081CME0080CME0100CME0094CME0077CME0056CME0101CME0117CME0128检测靶标灵敏度HumanHumanHumanHumanMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouse反应种属检测范围规格产品编号2526定制化服务产品表Adiponectin/Acrp30(脂联素)CCL20/MIP-3alpha(趋化因子CCL20)CINC-1/CXCL1(细胞因子诱导的中性粒细胞趋化因子1)CNTF(睫状神经营养因子)CXCL3/CINC-2(趋化因子CXCL3)IgA(免疫球蛋白A)L-Selectin/CD62L(L-选择素)TIM-1/KIM-1/HAVCR(T细胞免疫球蛋白粘蛋白1)CRP(C反应蛋白)IL-1α/IL-1F1(白细胞介素1α)Phospho-Akt(S473)(磷酸化Akt(S473))Phospho-ERK1(T202/T204)(磷酸化ERK1)Phospho-ERK1(T202/T204)(磷酸化ERK1)Phospho-ERK2(T185/Y187)(磷酸化ERK2)TotalHIF-2alpha/EPAS1(总低氧诱导因子2α)80pg/mL15pg/mL7pg/mL15pg/mL15pg/mL8ng/mL31pg/mL7pg/mL40pg/mL50pg/mL80pg/mL60pg/mL235pg/mL235pg/mL60pg/mL96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells156~10,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL31.
20~2,000pg/mL16~1,000ng/mL62.
50~4,000pg/mL15.
60~1,000pg/mL78.
10~5,000pg/mL93.
80~6,000pg/mL156~10,000pg/mL125~8,000pg/mL469~30,000pg/mL469~30,000pg/mL125~8,000pg/mLCRE0027CRE0023CRE0013CRE0022CRE0024CRE0014CRE0032CRE0028CSE0007CSE0009CSPE0006CSPE0007CSPE0003CSPE0003CSPE0004灵敏度RatRatRatRatRatRatRatRatSwineSwineOtherOtherOtherOtherHuman/Rat反应种属检测范围规格产品编号检测靶标ELISA试剂盒售前售后服务TECHNICALSUPPORT【ELISA售前咨询】若有任何四正柏ELISA产品技术咨询,请电邮至tech@4abio.
com【ELISA售后服务】若有任何四正柏产品使用问题反馈,可直接通过以下链接在线填写信息,我们将第一时间为您处理.
http://market-4abio-tech.
mikecrm.
com/pICnnhpELISA试剂盒检测服务ELISATESTINGSERVICE【收费标准】请咨询四正柏业务人员.
【提供结果】提供正式实验报告,含标准曲线图.
【服务周期】5~7天,取决于是否需做预实验、样本及待测指标的数量.
【样本要求】人和动物的血清、血浆及细胞培养上清,-20℃保存,避免反复冻融.
引用产品:MouseTNF-αELISAKit作者单位:武汉大学化学与分子科学学院期刊影响因子:21.
95【01】Chu-XinLi,YuZhang,XueDong,etc.
ArtificiallyReprogrammedMacrophagesasTumor‐TropicImmunosuppression‐ResistantBiologicstoRealizeTherapeuticsProductionandImmuneActivation.
AdvancedMaterialsFirstpublished:25February2019.
引用产品:InsulinELISAkits作者单位:武汉大学化学与分子科学学院期刊影响因子:21.
95【02】Di-WeiZheng,LuXu,Chu‐XinLi,etc.
Photo‐PoweredArtificialOrganellesforATPGenerationandLife‐Sustainment.
AdvancedMaterialsFirstpublished:31October2018.
(IF:21.
95).
【03】LinGuo,LihuiLin,DuanqingPei,etc.
ResolvingCellFateDecisionsduringSomaticCellReprogrammingbySingle-CellRNA-Seq.
MolecularCellVolume73,Issue4,21February2019,Pages815-829.
e7.
引用产品:MouseIFN-γELISAKit作者单位:中国科学院广州生物医学与健康研究所再生生物学重点实验室期刊影响因子:14.
248【04】DingkaSong,JunchenMeng,JieCheng,etc.
Pseudo-monasaeruginosaquorum-sensingmetaboliteinduceshostimmunecelldeaththroughcellsurfacelipiddomaindissolution.
NatureMicrobiologyNov15,2018,BSR20181501.
引用产品:HumanIL-8ELISAKit作者单位:清华大学生命科学中心基础医学系免疫学研究所期刊影响因子:14.
174【05】Jin-YueZeng,Mei-ZhenZou,MingkangZhang,etc.
π-ExtendedBenzoporphyrin-BasedMetal–OrganicFrameworkforInhibitionofTumorMetastasis.
ACSNano2018,12(5),pp4630–4640.
引用产品:MouseTNF-αandIFN-γELISAkit作者单位:武汉大学化学与分子科学学院期刊影响因子:13.
942【06】Rong-HuiDeng,Mei-ZhenZou,DiweiZheng,etc.
NanoparticlesfromCuttlefishInkInhibitTumorGrowthbySynergizingImmunotherapyandPhotothermalTherapy.
ACSNano2019PublicationDate:June27,2019.
引用产品:MouseTNF-α,IL-6,IL-12p40,IFN-γELISAKits作者单位:武汉大学化学与分子科学学院期刊影响因子:13.
903【07】WenbiaoWang,GengLi,DeWu,etc.
ZikavirusinfectioninduceshostinflammatoryresponsesbyfacilitatingNLRP3inflammasomeassemblyandinterleukin-1βsecre-tion.
NatureCommunications9,Articlenumber:106(2018).
引用产品:MouseIL-1βELISAKit作者单位:武汉大学病毒学国家重点实验室和生命科学学院期刊影响因子:12.
124【08】FionaMoghaddas,PingZeng,YuxiaZhang,etc.
AutoinflammatorymutationinNLRC4revealsaleucine-richrepeat(LRR)–LRRoligomerizationinterface.
JournalofAllergyandClinicalImmunologyVolume142,Issue6,December2018,Pages1956-1967.
e6.
引用产品:HumanIL-1βandIL-18ELISAKits作者单位:澳大利亚帕克维尔沃尔特和伊莱莎霍尔医学研究所期刊影响因子:13.
258SCI文献引用列表(部分)PUBLICATIONSLIST(PART)2728【09】FanGao,ChiZhang,Wen‐XiuQiu,etc.
PD‐1BlockadeforImprovingtheAntitumorEfficiencyofPolymer–DoxorubicinNanoprodrug.
SmallFirstpublished:20August2018.
引用产品:MouseIFN-γandTNF-αELISAkits作者单位:武汉大学化学与分子科学学院期刊影响因子:9.
598【10】FengZhang,QiangZhang,XiangyangLi,etc.
Mussel-inspireddopamine-CuIIcoatingsforsustainedinsitugenerationofnitricoxideforpreventionofstentthrombosisandrestenosis.
BiomaterialsVolume194,February2019,Pages117-129.
(IF:8.
806).
引用产品:HumanSerpinE1/PAI-1andvWF-A2ELISAKits作者单位:西南交通大学材料科学与工程学院教育部材料先进技术重点实验室期刊影响因子:8.
806【11】Tong-FeiLi,KeLi,QuanZhang,etc.
Dendriticcell-mediateddeliveryofdoxorubicin-polyglycerol-nanodiamondcompositeselicitsenhancedanti-cancerimmuneresponseinglioblastoma.
Biomate-rialsVolume181,October2018,Pages35-52.
引用产品:HumanIL-12p40,MouseIFN-γandIL-2ELISAkits作者单位:武汉大学医学部期刊影响因子:8.
8【15】E.
Wang,X.
Liu,W.
Tu,etc.
Benzo(a)pyreneFacilitatesDermatophagoidesGroup1(Derf1)‐InducedEpithelialCytokineReleasethroughArylHydrocarbonReceptorinAsthma.
AllergyFirstpublished:14April2019.
引用产品:MouseIL-4,IL-13,IFNγ,IL-17A,TSLP,IL-33,andIL-25ELISAKits作者单位:深圳大学附属罗湖医院期刊影响因子:6.
23【16】XiaochengZhou,TinglinYan,ChunmingHuang,etc.
Melanomacell-secretedexosomalmiR-155-5pinduceproangio-genicswitchofcancer-associatedfibroblastsviaSOCS1/-JAK2/STAT3signalingpathway.
JournalofExperimental&ClinicalCancerResearch201837:242.
引用产品:MouseVEGF,FGF-basic,andMMP9ELISAkits作者单位:武汉大学口腔医学院期刊影响因子:6.
217【12】Mei‐ZhenZou,Wen‐LongLiu,Chu‐XinLi,etc.
AMultifunctionalBiomimeticNanoplatformforRelievingHypoxiatoEnhanceChemotherapyandInhibitthePD‐1/PD‐L1Axis.
Small2018,1801120.
引用产品:MouseTNF-α,IL-6,IL-12,IFN-rELISAKit作者单位:武汉大学化学与分子科学学院期刊影响因子:8.
643【13】Wen-XiuQiu,Ming-KangZhang,Li-HanLiu.
Aself-deliverymembranesystemforenhancedanti-tumortherapy.
BiomaterialsVolume161,April2018,Pages81-94.
引用产品:MouseTNF-α,IFN-γ,IL-1β,IL-12p70ELISAKit作者单位:武汉大学生物医学高分子教育部重点实验室期刊影响因子:8.
402【17】FanGao,QianCheng,Miao-DengLiu,etc.
LocalTregulatorycellsdepletionbyanintegratednanodrugsystemforefficientchem-immunotherapyoftumor.
ScienceChina-chemis-tryPublishedJun27,2019.
引用产品:MouseTNF-αandIL-10ELISAKits作者单位:武汉大学化学与分子科学学院期刊影响因子:6.
085【18】DiWu,ChuanTang,YanfangLiu,etc.
Structuralelucida-tionandimmunomodulatoryactivityofaβ-D-glucanpreparedbyfreeze-thawingfromHericiumerinaceus.
CarbohydratePolymersVolume222,15October2019,114996.
引用产品:MouseTNF-α,IL-1βandIL-6ELISAKits作者单位:上海农业科学院食用菌研究所期刊影响因子:6.
044【19】WuSun,XiaojunWang,JialuLi,etc.
MicroRNA-181apromotesangiogenesisincolorectalcancerbytargetingSRCIN1topromotetheSRC/VEGFsignalingpathway.
CellDeath&Disease9,Articlenumber:438(2018).
引用产品:HumanVEGFELISAkit作者单位:天津医科大学肿瘤研究所期刊影响因子:5.
965【20】ChaoJiang,PingXie,RuxuSun,etc.
c-Jun-mediatedmicroRNA-302d-3pinducesRPEdedifferentiationbytargetingp21Waf1/Cip1.
CellDeath&Disease9,Articlenumber:451(2018).
(IF:5.
965).
引用产品:HumanVEGF-AELISAkit作者单位:南京医科大学附属第一医院眼科期刊影响因子:5.
965【21】NingboXia,TaifangZhou,XiaohanLiang,etc.
ALactateFermentationMutantofToxoplasmaStimulatesProtectiveImmunityAgainstAcuteandChronicToxoplasmosis.
FrontiersinImmunology2018;9:1814.
引用产品:MouseIL12p70,INF-γ,IL-10,andTNF-αELISAKits作者单位:华中农业大学农业微生物学国家重点实验室期刊影响因子:5.
511【22】FangX.
,ZhangT.
,YangM.
,etc.
HighCirculatingAlarinLevelsAreAssociatedwithPresenceofMetabolicSyndrome.
CellularPhysiologyandBiochemistry2018;51:2041–2051.
引用产品:HumanTNF-αELISAKit作者单位:重庆医科大学附属第二医院内分泌科期刊影响因子:5.
5【23】QiuyingZhao,QiaozhiWang,JiutaiWang,etc.
Maternalimmuneactivation-inducedPPARγ-dependentdysfunctionofmicrogliaassociatedwithneurogenicimpairmentandaberrantpostnatalbehaviorsinoffspring.
NeurobiologyofDiseaseVolume125,May2019,Pages1-13.
引用产品:HumanIL-6andTNF-αELISAKits作者单位:中国电子科技大学信息生物学中心生命科学与技术学院期刊影响因子:5.
227【24】Wen-diLuo,Jia-weiMin,Wen-XianHuang,etc.
VitexinreducesepilepsyafterhypoxicischemiaintheneonatalbrainviainhibitionofNKCC1.
JournalofNeuroinflammation201815:186.
引用产品:RatIL-1β,IL-6,andTNFELISAKit作者单位:武汉大学基础医学院期刊影响因子:5.
193【25】Xing-LiangYang,XinWang,LinShao,etc.
TRPV1mediatesastrocyteactivationandinterleukin-1βreleaseinducedbyhypoxicischemia(HI).
JournalofNeuroinflammation201916:114.
引用产品:MouseIL-1βELISAKit作者单位:武汉大学基础医学院湖北省发育源性疾病重点实验室生理学教研室期刊影响因子:5.
193【26】Rui-ChengYang,Xin-YiQu,Si-YuXiao,etc.
MeningiticEscherichiacoli-inducedupregulationofPDGF-BandICAM-1aggravatesblood-brainbarrierdisruptionandneuroinflammatoryresponse.
JournalofNeuroinflammation201916:101.
引用产品:MousePDGF-BBELISAKit作者单位:华中农业大学可持续猪生产合作创新中心期刊影响因子:5.
193【14】Xiao-YanHe,Bo-YaLiu,ChangXu,etc.
Amulti-function-almacrophageandtumortargetinggenedeliverysystemfortheregulationofmacrophagepolarityandreversalofcancerimmunoresistance.
Nanoscale2018,10,15578-15587.
引用产品:MouseIL-12p40,IL-6,IL-10,IL-4,TNF-αELISAKits作者单位:武汉大学化学系生物医学聚合物教育部重点实验室期刊影响因子:7.
2332930
4abio.
netHRPTMB北京(总部)北京市经济技术开发区科创十四街20号院1号楼103室TEL:010-67885057E-mail:info@4abio.
com上海上海市徐汇区桂平路471号桂果园9号楼602室TEL:021-51001750E-mail:shanghai@4abio.
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com苏州苏州市高新区科技城锦峰路8号17号楼201室E-mail:suzhou@4abio.
comELISAKIT.
ElisaTechnicalGuideandProducts.
www.
4abio.
net目录CONTENTS【背景篇】---01~02/关于我们01~02/四正柏与ELISA的故事03~03/四正柏全国奖励金计划0104【技术篇】---04~04/ELISA技术简介05~06/常见的ELISA类型07~08/ELISA样本制备指南09~10/ELISA实验常见问题解答11~12/品质管理标准13【产品篇】---13~17/人细胞因子ELISA试剂盒18~19/小鼠细胞因子ELISA试剂盒20~20/大鼠细胞因子ELISA试剂盒20~20/猪细胞因子ELISA试剂盒21~21/其他标志物ELISA试剂盒21~21/ELISA辅助试剂【附录】---27~30/SCI文献引用列表272226【服务篇】---22~25/ELISA试剂盒定制服务25~25/ELISA试剂盒检测服务26~26/ELISA试剂盒售前售后服务0201北京四正柏生物科技有限公司(Beijing4ABiotechCo.
,Ltd.
)创办于2009年,地处北京经济技术开发区,是一家集生物医药产品研发、生产、销售、技术服务四位一体的高新技术企业.
四正柏旗下有包括北京东方华辉生物医药在内的多家从属公司,业务涵盖生物医药实验室用品、技术服务、体外诊断试剂(IVD)三大板块.
公司凭借深厚悠久的行业合作背景,与国内外专家合作建成了多个技术平台,产品涉及分子生物学、细胞生物学、免疫学、血液学等领域,多年培育形成了以ELISA、流式细胞术为代表的特色技术优势.
四正柏生物在国内全面建成销售网络,在北上广深等地均设有经营实体,此外还在北美、欧洲等地建成销售渠道,产品销往海外并获市场高度认可.
四正柏生物始终坚持"掌握源头技术,专业创造价值"的发展理念,企业愿景是发展成为国内生命科学和临床诊断领域最具竞争力的高新技术企业,并在国际市场树立强有力的竞争形象.
「1992」年:晶美公司取得当时最大的细胞因子试剂供应商--美国Genzyme公司的独家代理权.
「1993」年:经Genzyme授权和培训,在国内首度分装其DuoSet品牌细胞因子试剂盒.
美方派出技术专家对工艺流程和所有组分的质控(包括包板、干板技术、标本稀释液中基质效应的控制、酶标板选择、试剂瓶防漏测试、标签设计等等)进行了严格的培训和监控,分装产品迅速取得了商业上的成功.
在其后的20年里,国内出现了数十家分装企业,形成了一个独特的区隔市场,而晶美公司一直是这个市场的领导者.
「1998」年:因为竞购流式细胞仪抗体试剂公司PharMingen失利,Genzyme公司放弃科研试剂业务,把细胞因子试剂部门卖给R&DSystem公司.
晶美公司继续代理R&DSystem在中国的销售,直到2008年底.
「2009」年:四正柏生物在原晶美公司研发生产中心的基础上成立,专注于单克隆抗体和细胞因子试剂的开发、生产和相关服务.
经过10年的发展,目前可提供经过国际标准质控的300多种ELISA试剂盒,以极具竞争力的性价比销往全球市场.
27年专注ELISA实验解决方案StorywithELISA四正柏与ELISA的故事Aboutus----ELISAKIT四正柏全国奖励金计划SCHOLARSHIPPROGRAM洗涤除去未结合试剂用捕获抗体包被微孔板封闭洗涤除去未结合试剂加待检样本孵育加生物素化检测抗体孵育加HRP酶-链亲和素结合洗涤弃未结合的检测抗体洗涤除去未结合试剂加终止液并读数加显色底物酶联免疫吸附实验(ELISA)是一种实验室常用的检测方法,广泛应用于测量溶液中的分析物(通常是抗体或抗原)的浓度.
与其他基于抗体的检测方法不同的是,酶联免疫吸附实验(ELISA)或酶免疫测定(ELISA)通过与固相支持物(通常为聚乙烯多孔板)的结合和系列洗涤步骤,实现特异性和非特异性相互作用的分离,并可通过最终有色产物的形成,定量分析原始样品中分析物体的含量.
ELISA实验快速且简单,可迅速分析大量平行的样品,在基础研究和临床诊断实践中广泛使用.
/基本概述Introduction申请方式点击链接http://cn.
mikecrm.
com/3UncvoH或扫描在右方二维码,填写资料并上传PDF全文即可参与.
ELISA实验始于包被,即将抗原或抗体吸附到聚苯乙烯孔板中(吸附通常是溶液被动附着于固体表面上产生一层薄膜),包被后为封闭和检测步骤等(如右简图).
由于ELISA是通过与固相支持物的结合来实现分离分析,因此每个操作步骤间均有重复的洗板操作,以去除未结合的物质.
在此过程中,最关键的就是要充分去除残余溶液,以防止稀释后续步骤加入的试剂.
要获得最佳的一致性结果,可使用自动洗板机来完成洗涤步骤.
ELISA实验虽然原理简单,但仍需谨慎认真对待.
如样品中一些干扰物质的存在,孵育温度的变化等均可影响实验结果.
其中最关键的步骤当属检测环节,因其涉及最终的信号放大以及结果读取.
/基本流程Workow「柏」花点1点=100元奖励金,可兑换现金或产品抵扣券,用于赞助学术会议注册或差旅费.
可以课题组为单位进行累积,有效期为两年.
【IF.
作为民族品牌、高端生物试剂国产化领先者,四正柏通过近十年时间坚持走自主创新之路,建设了数条颇具竞争力的产品线,受到全球科研工作者的青睐.
/奖励条件Requirements/活动概述Introduction/奖励方案RewardProgram/【柏】花点使用方法InstructionsA.
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0304ELISA技术简介IntroductiontoELISAPrinciples常见的ELISA类型TYPESOFELISA直接法主要用于测定抗原间接法主要用于测定抗体0506ELISA可用于测定抗原,也可用于测定抗体.
通常可适用于测定细胞培养上清、血清、血浆以及组织裂解物样品等.
在ELISA检测方法中有三种必要的试剂:①固相的抗原或抗体;②酶标记的抗原或抗体;③酶反应底物.
根据试剂的来源和样品的性质以及检测的实验条件,可设计出各种不同类型的ELISA检测方法.
双抗体夹心法主要用于测定抗原将抗原与固相载体连接,洗涤除去未结合的抗原及杂质.
加酶标抗体进行孵育,固相免疫复合物上的抗原与酶标抗体结合,彻底洗涤未结合的酶标抗体,此时固相上带有的酶量与标本中受检抗原的量相关,加底物反应.
【优点】操作流程简短,实验步骤少;无需使用二抗避免了交叉反应;实验步骤少,操作不易出错;【缺点】实验中的一抗均需直接酶标记,成本相对较高.
/直接法DirectELISA将特异性抗体(通常称为捕获抗体)与固相载体连接,形成固相抗体.
洗涤除去未结合的抗体及杂质.
加入待检样品,恒温孵育.
样品中的特异性抗原与固相抗体结合,形成固相抗原抗体复合物.
洗涤除去其它未结合物质.
加酶标抗体(通常称为检测抗体),保温孵育.
固相免疫复合物上的抗原与酶标抗体结合.
彻底洗涤未结合的酶标抗体.
加底物反应.
高特异性,使用的捕获抗体和检测抗体都是检测物特异性适用于复杂样品,抗原不需进行纯化即可用于检测灵活性更大,灵敏度更高,可采用直接法也可采用间接法.
检测物需要拥有两个以上不同的抗原表位或多个相同的重复表位;不适用于检测小分子物质.
/双抗体夹心法SandwichELISA酶标二抗可加强信号,提高灵敏度灵活性更大,同一酶标二抗可应用于多种不同的一抗不直标一抗,可保留其最多的免疫反应性成本更低,使用的标记抗体更少.
【优点】将抗原与固相载体连结,形成固相抗原.
洗涤除去未结合的抗原及杂质.
加入特异性一抗与固相抗原结合,形成固相抗原抗体复合物.
经洗涤后,加酶标二抗.
固相免疫复合物中的抗体与酶标二抗结合,从而间接地标记上酶.
洗涤后,加底物显色.
【缺点】交叉反应几率升高.
/间接法IndirectELISA竞争法ELISA最为复杂,通常用于检测小分子如半抗原、激素、药物等.
样品中待检游离抗原与固定于固相载体上的抗原一起竞争相同的有限量抗体,当样品中的游离抗原越多,就可结合越多的抗体,而固相抗原则只能结合较少的抗体.
反之亦然.
经洗涤去除样品中抗原与抗体的结合物,只留下固相抗原与抗体的结合物.
显色经计算可得样品中抗原的含量.
竞争法也可根据实验设置直接法,间接法或夹心法形式.
/竞争法CompetitiveELISA无信号表明无带标记的抗体结合到固相载体,样品中抗原的含量很高检测抗体与样品中抗原结合,经洗涤被除去用已知抗原包被固相载体然后封闭加入含未知抗原的样品加入带标记的检测抗体ELISA样本制备指南ELISASAMPLEPREPARATIONGUIDE【细胞培养上清】将细胞培养基移至离心管,并在4℃条件下以1,500rpm离心10min.
立即等分上清液并于-80℃下保存样品.
尽可能减少样品冻融.
【血清】室温血液自然凝固10~20min后,4℃条件下以3,000rpm离心10min.
立即等分上清液并于-80℃下保存样品.
保存过程中如有沉淀形成,应再次离心.
避免反复冻融样品.
【血浆】将全血收集到含抗凝血剂的管中,根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,在4℃条件下以3,000rpm离心10分钟.
立即等分上清液(血浆)并于-80℃下保存样品,避免反复冻融样品.
【唾液】收集样品并在4℃下以10,000xg离心2分钟.
立即等分上清液并在-80℃下保存样品,避免反复冻融样品.
【尿液】用无菌管收集,并在4℃下以10,000xg离心2分钟.
立即等分上清液并于-80℃下保存样品.
避免反复冻融样品.
采集组织(最好在冰上操作,并应尽可能快以防止被蛋白酶降解)样品后,称取组织净重,用液氮迅速冷冻保存备用.
将样品保存在-80℃下以便后续使用或放置在冰上进行直接匀浆.
加入一定量的组织提取缓冲液,用手工或匀浆器将样品匀浆充分.
在4℃下以13000rpm离心20分钟.
置于冰上,将上清液(可溶的蛋白质提取物)等分至新的冷却管中并在-80℃下保存样品.
避免反复冻融样品.
【液体类型样品】液体类实验样品:血清、血浆、尿液、胸腹水、细胞培养上清等【组织样品】08该指南旨在作为教育资源,指南中提供的各种制备方法适用于常规ELISA样品的制备方法通常也会随测定靶标和检测方法而适当变化在建立新的检测方法时,建议查阅相关文献或进行必要的流程优化在收集样品前有必要建立完整的计划,必须清楚要检测的成份是否足够稳定我们提倡新鲜样品尽早检测对收集后当天就进行检测的标本及时储存在4℃备用如有特殊原因需要周期性收集样品在造模取材后,将标本及时分装后放在-20℃或-80℃条件下保存因冰箱与室温存在一定温差,蛋白极易降解,直接影响实验质量,所以避免反复冻融07操作基本流程WorkowELISA实验常见问题解答将洗涤缓冲液加入孔中洗涤,确保孔区域洗涤充分;确保已在洗涤前弃去所有残留抗体溶液;增加洗涤次数;检查偶联物的稀释度,在推荐的稀释度下使用;检查封闭液;延长封闭时间;缩短孵育时间;设置适当对照;新鲜配置缓冲液;重复实验检查试剂配制方法;复核试验试剂添加流程;使用新鲜配制的试剂;检查是否使用了推荐的抗体量;增加抗体用量(浓度);检查使用的试剂;使用ELISA板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;新鲜配置缓冲液;严格按照说明书洗涤流程操作;底物溶液混合后须立即使用;每步均需使用新的封板胶纸或试剂储液槽;新鲜配置缓冲液;检查倍性稀释是否正确;使用酶标板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;检查稀释度是否正确;延长底物溶液孵育时间;严格按照说明书操作流程;重新计算制作新的标准曲线;将洗涤缓冲液加入孔中洗涤,确保孔区域洗涤充分;确保已在洗涤前弃去所有残留溶液;检查封闭步骤是否正确;使用酶标板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;每步均需更换使用新的封板胶纸;须使用封板胶纸;新鲜配置缓冲液;洗板不充分链亲和素-HRP结合物过量孵育时间过长样品或标准品中含干扰物质缓冲液受污染HRP被叠氮化合物污染使用了含胎牛血清的缓冲液复溶抗体缓冲液受污染洗板不充分或跳过洗涤步骤未去除残留的非结合过氧化物酶底物溶液预混太早不新鲜封板胶纸或试剂储液槽重复使用导致HRP残留污染缓冲液有金属或HRP污染链亲和素-HRP结合物量不足检测抗体量不足孵育时间不足实验操作流程有误标准曲线稀释计算错误重复使用封板胶纸未使用封板胶纸缓冲液受污染试剂添加顺序错误或试剂配制不正确封闭不充分抗体用量不足捕获抗体与板结合较差捕获抗体与板结合较差洗板不充分酶标板包被不均匀Part01高背景信号Part02无信号Part05板内重复性差Part03整板呈现规则蓝色Part04标准曲线不佳梯度不明显(值低或曲线平直)0910解决方法可能原因问题解决方法将洗涤缓冲液加入孔中洗涤,确保孔区域洗涤充分;确保已在洗涤前弃去所有残留抗体溶液;严格按照推荐的温度孵育;勿在环境温度变化大的地方孵育;严格按照相同的操作流程;每步均需更换使用新的封板胶纸;新鲜配置缓冲液;设置阳性对照;重复实验;做系列稀释检测回收率;稀释样品重新实验;检查酶标仪;延长显色时间;重新包被新的酶标板;使用酶标板,而非组织培养板;使用不含其他杂蛋白的PBS溶解抗体;检查使用的试剂;孵育时使用封板胶纸;勿在环境温度变化大的地方孵育;确保使用前酶标板处于室温下且试剂也处于室温下;避免使用含这些防腐剂的试剂;Part06板间重复性差Part07标准曲线佳但样品孔无信号Part09整板读数偏低Part10边缘效应Part11显色缓慢问题可能原因洗板不充分操作流程改变封板胶纸重复使用导致HRP残留污染缓冲液受污染样品基质掩盖检测样品中检测物超过标准曲线范围酶标仪波长设置不正确显色时间不充分包被的酶标板超过使用效期使用了含胎牛血清的缓冲液复溶抗体酶标板未处于正确温度下溶液污染孵育温度不均衡捕获抗体与板结合较差孵育温度发生变化样品中无物体检测物或表达量极低Part08标准曲线佳但样品信号偏高ELISATroubleshooting1112四正柏自主品牌产品SCI文献引用(数据截至2019年6月30日)80060040020007302019年6082018年4712017年3542016年2622015年1832014年1162013年682012年282011年62010年四正柏ELISA试剂盒如何确保高品质四正柏ELISA试剂盒的所有生产环节均采用严苛的质量控制标准.
从抗体对的筛选、酶标板的选择、包板、干板、样本稀释液中基质效应的控制、酶底物筛选、试剂瓶防漏测试等环节均经过多重严格的监控,确保提供的每一个试剂盒具有最佳的性能和一致性.
四正柏所有ELISA试剂盒均为完整的、经过验证的即用型试剂盒,可用于定量检测多种复杂样本类型中的靶蛋白含量.
试剂盒采用双抗夹心法免疫检测原理.
甄选全球优质单克隆抗体,利用公司先进的抗体生产和验证平台,进行严苛的抗体对筛选验证.
对多种候选单抗及多抗组合进行差异性测试,严选最佳抗体对组合用于试剂盒开发.
对选择的抗体进行仔细地滴定以确保所选择的浓度可获得最好的检测结果.
在包板过程中,捕获抗体以不同的浓度梯度包被于微孔板,以测试出捕获抗体的最佳结合力和精确度.
检测抗体同样以不同浓度进行测试,以确保最佳浓度下检测抗体与捕获抗体的最佳配对,获得最佳检测信噪比,确保试剂盒高度特异性的检测靶蛋白,具备极高的稳定性.
严格的抗体对筛选标准精密度是试剂盒重复实验时误差大小的评估标准,通常用变异系数CV值表示.
该值是试剂盒的关键性能指标.
A.
可确保多次实验结果的准确性和可重复性;B.
确定两次实验结果是否相同或不同.
变异系数CV值通常需要考虑板内(intra-assay)精密度及板间(inter-assay)精密度.
板内CV值反应同一微孔板上不同板孔间的检测重复性,研究人员可在同一张板上设置复孔测试样品,获得相近结果;板间CV值反应不同批次间不同微孔板的检测重复性,研究人员可在一个较长的时间段使用不同批次试剂盒时获得重复性较好的结果.
四正柏公司所有ELISA试剂盒的板间和板内CV值都控制在10%以内.
精密度与重复性特异性一般指试剂盒出现假阳性和假阴性的概率,也指试剂盒对同类物质的交叉反应率.
抗体交叉反应及基质干扰因素都会影响试剂盒的特异性.
当某种非目标分析物分子与抗体对结合时,发生交叉反应则导致假阳性结果.
当样本基质中某些物质干扰目标分析物与抗体结合时,则发生干扰现象导致假阴性结合.
要防止以上问题的发生,必须经过系列严格的开发和测试过程.
四正柏ELISA试剂盒均会检测与目标检测物同家族的其他蛋白、其他种属相同靶点蛋白以及血清中常见因子的交叉反应率.
同时还会对靶蛋白配体或靶蛋白受体的干扰因素进行测试.
交叉反应及干扰性越小,说明试剂盒的特异性越高.
特异性回收率是试剂盒准确性的判断标准.
复杂样本如血清和血浆等,可能含有干扰因子而影响目标分析物的检测准确性.
添加高、中、低三种浓度的待检目标物标准品到天然样本基质中进行测定,以不添加标准品蛋白的天然样本基质为空白对照,加标的样本所得的结果减去未加标对照样本所得的结果,其差值同加入标准物质的理论值之比即为样品加标回收率.
四正柏ELISA试剂盒的回收率标准是80-120%以内视为合格.
回收率线性是试剂盒准确度和特异性的判断标准.
干扰因子会影响检测的线性,试剂盒需经过严格优化.
四正柏选用高浓度的天然样本或重组蛋白在试剂盒的检测范围内进行梯度稀释,分别测定各浓度下的回收率.
回收率在80-120%之间视为合格.
稀释线性最低检测限是指以恰当的置信概率被检测出的目标分析物的最低浓度值.
一般通过做20个空白对照,最低检测限即该20个空白对照孔OD值的平均值2SD(20个空白对照孔2倍的标准偏差).
最低检测限的值越低说明试剂盒越灵敏.
灵敏度四正柏提供300余种高品质ELISA试剂盒灵敏度高批间差小重复性佳传承27年ELISA开发经验27+YEARSOFELISAMANUFACTURINGEXPERIENCE目录产品表CATALOGUEELISAKITSCXCL16(趋化因子CXCL16)CXCL2/GROβ(人趋化因子CXCL12)CXCL4/PF4(趋化因子CXCL4)CXCL5/ENA-78(趋化因子CXCL5)CXCL6/GCP-2(趋化因子CXCL6)CXCL7/NAP-2(趋化因子CXCL7)DDR1(盘状结构域受体酪氨酸激酶)DPPIV/CD26(二肽酰多肽酶)EGF(表皮生长因子)eNOS(内皮型一氧化氮合酶)FasLigand/TNFSF6(Fas配体)Fas/Apo-1(凋亡相关因子)FetuinA/AHSG(人胎球蛋白A)FGFacidic(酸性成纤维生长因子)FGFbasic(碱性成纤维生长因子)FGF-19(成纤维生长因子19)FGF-21(成纤维生长因子21)FGF-23(成纤维生长因子23)Galectin-3(半乳凝素)G-CSF(粒细胞集落刺激因子)GDF-15(生长分化因子15)GDNF(胶质细胞源性神经营养因子)GM-CSF(粒细胞巨噬细胞集落刺激因子)GranzymeA(人粒酶A)GranzymeB(粒酶B)HB-EGF(肝素结合表皮生长因子)Hepcidin(铁调素)HGF(肝细胞生长因子)HSP60(热休克蛋白60)HSP70/HSPA1A(热休克蛋白70)IFN-α(α干扰素)IFN-β(人β干扰素)IFN-γ(γ干扰素)IgA(免疫球蛋白A)IgE(人免疫球蛋白E)IGFBP-1(胰岛素样生长因子结合蛋白1)IGFBP-7IGFBP-rp1(人胰岛素样生长因子结合蛋白-7)IGF-I(胰岛素样生长因子1)IgGtotal(总免疫球蛋白G)IgM(免疫球蛋白M)IL-1R4/ST2(白细胞介素1受体4)IL-10(白细胞介素10)IL-11(白细胞介素11)7pg/mL7pg/mL7pg/mL0.
5pg/mL7pg/mL7pg/mL78pg/mL7pg/mL15pg/mL40pg/mL15pg/mL70pg/mL15pg/mL60pg/mL30pg/mL7pg/mL15pg/mL40pg/mL31pg/mL4pg/mL4pg/mL15pg/mL7pg/mL15pg/mL20pg/mL4pg/mL4pg/mL60pg/mL300pg/mL60pg/mL7pg/mL4pg/mL7pg/mL0.
8ng/mL234pg/mL15pg/mL39pg/mL39pg/mL15pg/mL0.
8ng/mL7ng/mL15pg/mL7pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells15.
60~1,000pg/mL15.
625~1000pg/mL15.
60~1,000pg/mL1.
95~125pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL156.
00~10000pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL78.
10~5,000pg/mL31.
20~2,000pg/mL156~10,000pg/mL31.
25~2000pg/mL125.
00~8,000pg/mL64~4,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL78.
10~5,000pg/mL62.
50~4,000pg/mL7.
80~500pg/mL7.
8~500pg/mL31.
25~2,000pg/mL15.
60~1,000pg/mL31.
25~2000pg/mL39.
10~2,500pg/mL7.
81~500pg/mL7.
8~500pg/mL125~8,000pg/mL625.
00~40,000pg/mL125.
00~8,000pg/mL15.
60~1,000pg/mL7.
8~500pg/mL15.
60~1,000pg/mL1.
60~100ng/mL468.
75~30000pg/mL31.
20~2,000pg/mL78~5000pg/mL78~5000pg/mL31.
20~2,000pg/mL1.
60~100ng/mL15.
60~1,000ng/mL31.
20~2,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mLCHE0117CHE0278CHE0210CHE0162CHE0214CHE0212CHE0283CHE0131CHE0044CHE0115CHE0196CHE0024CHE0273CHE0202CHE0060CHE0201CHE0199CHE0175CHE0130CHE0025CHE0112CHE0046CHE0026CHE0275CHE0127CHE0144CHE0287CHE0069CHE0192CHE0193CHE0084CHE0274CHE0017CHE0093CHE0270CHE0070CHE0265CHE0265CHE0071CHE0095CHE0096CHE0036CHE0013CHE0010HOTHOTHOTHOTHOTHOTHOT人细胞因子ELISA试剂盒(HumanCytokineELISAKits)检测靶标灵敏度检测范围规格产品编号人细胞因子ELISA试剂盒(HumanCytokineELISAKits)ActivinA(激活素A)ADAMTS5(血小板反应蛋白解整合素金属肽酶5)Adiponectin(脂联素)Amphiregulin(双调蛋白)Angiopoietin-2(血管生成素2)Angiopoietin-like4(血管生成素类似物4)ApolipoproteinA-1/ApoA1(载脂蛋白A1)BAFF/TNFSF13B(B细胞活化因子)BDNF(脑源性神经营养因子)BMP-2(骨形态发生蛋白2)BMP-6(骨形态发生蛋白6)BMP-7(骨形态发生蛋白7)CCL1/I-309(趋化因子CCL1)CCL17/TARC(趋化因子CCL17)CCL19/MIP-3beta(趋化因子CCL19)CCL21(趋化因子CCL21)CCL22/MDC(趋化因子CCL22)CCL24/Eotaxin-2(趋化因子CCL24)CCL25/TECK(趋化因子CCL25)CCL26/Eotaxin-3(趋化因子CCL26)CD27/TNFRSF7(肿瘤坏死因子受体超家族成员7)ComplementComponentC5a(补体成分5a)Corin(丝酪氨酸蛋白酶)CRP(C反应蛋白)Cu/ZnSOD(铜锌超氧化物歧化酶)CXCL1/GRO(趋化因子CXCL1)CXCL10/IP10(趋化因子CXCL10)CXCL11/I-TAC(趋化因子CXCL11)CXCL12/SDF-1(趋化因子CXCL12)CXCL13/BCA-1/BLC(趋化因子CXCL13)CXCL14/BRAK(趋化因子CXCL14)60pg/mL62pg/mL7pg/mL7pg/mL50pg/mL0.
6ng/mL2ng/mL20pg/mL15pg/mL23pg/mL80pg/mL31pg/mL7pg/mL4pg/mL7pg/mL15pg/mL4pg/mL15pg/mL31pg/mL31pg/mL62pg/mL15pg/mL31pg/mL7pg/mL7pg/mL15pg/mL15pg/mL4pg/mL15pg/mL7pg/mL60pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells125~8,000pg/mL125~8000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL93.
80~6,000pg/mL1.
25~80ng/mL3.
12~200ng/mL39.
10~2,500pg/mL32.
50~2,000pg/mL46.
90~3,000pg/mL156.
00~10,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL15.
60~1,000pg/mL31.
25~2000pg/mL7.
81~500pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL62.
50~4,000pg/mL125~8000pg/mL31.
25~2000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL7.
81~500pg/mL31.
25~2,000pg/mL15.
60~1,000pg/mL125.
00~4,000pg/mLCHE0038CHE0292CHE0021CHE0150CHE0153CHE0154CHE0246CHE0076CHE0041CHE0078CHE0184CHE0182CHE0226CHE0220CHE0218CHE0140CHE0219CHE0224CHE0229CHE0222CHE0285CHE0296CHE0172CHE0104CHE0088CHE0065CHE0091CHE0213CHE0068CHE0211CHE0215检测靶标灵敏度检测范围规格产品编号HOT1314HOTIL-12p40(白细胞介素12p40)IL-12p70(白细胞介素12p70)IL-13(白细胞介素13)IL-15(白细胞介素15)IL-17(白细胞介素17)IL-17C(人白介素17C)IL-17E(人白介素17E)IL-18(白细胞介素18)IL-18BPaElisaKit(人IL-18结合蛋白alpha)IL-19(白细胞介素19)IL-1(白细胞介素1)IL-1α(白细胞介素1α)IL-2(白细胞介素2)IL-21(白细胞介素21)IL-22(白细胞介素22)IL-23(白细胞介素23)IL-24/MDA-7(白细胞介素24)IL-27(白细胞介素27)IL-28/IFN-lambda2(白细胞介素28)IL-29/IFNlambda1(白细胞介素29)IL-3(白细胞介素3)IL-31(白细胞介素31)IL-32α(白细胞介素32α)IL-33(白细胞介素33)IL-34(白细胞介素34)IL-36gamma/IL-1F9(白细胞介素36gamma)IL-37/IL-1F7(白细胞介素37)IL-4(白细胞介素4)IL-5(白细胞介素5)IL-6(白细胞介素6)IL-6Ralpha(白细胞介素6受体α)IL-6sRα(可溶性白细胞介素6受体α)IL-7(白细胞介素7)IL-8(白细胞介素8)IL-9(白细胞介素9)Irisin/FNDC5(鸢尾素)KGF/FGF-7(角化细胞生长因子)Ki-67/MKI67(增殖标记物Ki-67)Klotho(klotho跨膜蛋白)Leptin(瘦素)LIF(白血病抑制因子)lipocalin-2/NGAL(载脂蛋白2)MCP-1(单核细胞趋化蛋白1)MCP-2/CCL8(单核细胞趋化蛋白2)30pg/mL15pg/mL1pg/mL4pg/mL7pg/mL60pg/mL6pg/mL20pg/mL47pg/mL15pg/mL4pg/mL2pg/mL4pg/mL16pg/mL16pg/mL16pg/mL31pg/mL31pg/mL60pg/mL7pg/mL15pg/mL60pg/mL4pg/mL16pg/mL30pg/mL15pg/mL15pg/mL4pg/mL1pg/mL2pg/mL7pg/mL7pg/mL4pg/mL7pg/mL2pg/mL62pg/mL15pg/mL3pg/mL40pg/mL15pg/mL7pg/mL40pg/mL7pg/mL0.
8pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells62.
50~4,000pg/mL31.
25~2,000pg/mL3.
12~200pg/mL7.
81~500pg/mL15.
6~1,000pg/mL125.
00~8,000pg/mL11.
70~750pg/mL78~5,000pg/mL93.
75~6000pg/mL31.
25~2,000pg/mL7.
81~500pg/mL3.
91~250pg/mL7.
81~500pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL31.
25~2,000pg/mL62.
50~4,000pg/mL64~8,000pg/mL125~8,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL125~8,000pg/mL7.
81~500pg/mL32.
25~2,000pg/mL64~4,000pg/mL31.
25~2000pg/mL31.
20~2,000pg/mL7.
81~500pg/mL1.
60~100pg/mL3.
90~250pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL15.
60~1,000pg/mL3.
90~250pg/mL125~8000pg/mL31.
20~2,000pg/mL6.
25~400pg/mL39~2500pg/ml31.
25~2,000pg/mL15.
60~1,000pg/mL78~5,000pg/mL15.
60~1,000pg/mL1.
56~100pg/mLCHE0016CHE0015CHE0004CHE0055CHE0054CHE0251CHE0252CHE0007CHE0289CHE0018CHE0001CHE0101CHE0003CHE0056CHE0057CHE0008CHE0085CHE0022CHE0030CHE0031CHE0028CHE0032CHE0058CHE0034CHE0037CHE0301CHE0174CHE0005CHE0006CHE0009CHE0204CHE0261CHE0143CHE0011CHE0012CHE0303CHE0232CHE0198CHE0129CHE0053CHE0083CHE0121CHE0103CHE0062人细胞因子ELISA试剂盒(HumanCytokineELISAKits)检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOTHOTHOTHOTHOTHOTHOTHOTHOT人细胞因子ELISA试剂盒(HumanCytokineELISAKits)HOTMCP-3(单核细胞趋化蛋白3)M-CSF(巨噬细胞集落刺激因子)MIF(巨噬细胞移动抑制因子)MIG/CXCL9(γ干扰素诱导单核细胞因子)MIP-3α/CCL20(巨噬细胞炎性蛋白3α)MMP-13(基质金属蛋白酶13)MMP-2(基质金属蛋白酶2)MMP-3(基质金属蛋白酶3)MMP-9(基质金属蛋白酶9)MyD88(髓分化因子88)Myeloperoxidase(髓过氧化物酶)β-NGF(β-神经生长因子)OPG(护骨素)OSM-R(抑瘤素M受体)OsteepontinOPN(骨桥蛋白)Park7/DJ-1(帕金森氏病蛋白7)PBEF/Visfatin(前B细胞克隆增强因子)PD-1(程序性细胞死亡因子受体1)PDGF-AA(血小板衍化生长因子AA)PDGF-AB(血小板衍化生长因子AB)PDGF-BB(血小板衍化生长因子BB)PDGF-DD(血小板衍化生长因子DD)Periostin/OSF-2(成骨细胞特异性因子2)Peroxiredoxin2(人过氧化物酶2)PIGF(胎盘生长因子)Pro-CollagenIalpha1(I型胶原α1前体)Proinsulin(胰岛素原)Prolactin(催乳素)P-selectin(P选择素)Rantes(调节活化的正常T细胞表达和分泌因子)RBP4(视黄醇结合蛋白4)Resistin(抵抗素)S100B(S100B蛋白)sCD40L(可溶性CD40配体)SerpinE1/PAI-1(纤溶酶原激活物抑制剂1)sE-selectin(可溶性E-选择素)sFRP-5(分泌性卷曲相关蛋白)sICAM-1(可溶性细胞间粘附分子1)sIL-2R(白细胞介素2可溶性受体)sTNFRI/TNFRSF1A(可溶性肿瘤坏死因子受体)sTNFRII/TNFRSF1B(可溶性肿瘤坏死因子受体)Syndecan-1(多配体蛋白聚糖1)TGF-α(转化生长因子α)TGF-β1(转化生长因子β1)4pg/mL7pg/mL15pg/mL31pg/mL7pg/mL31pg/mL0.
31pg/mL15pg/mL30pg/mL78pg/mL31pg/mL15pg/mL15pg/mL80pg/mL30pg/mL32pg/mL2ng/mL80pg/mL7pg/mL7pg/mL15pg/mL4pg/mL30pg/mL390pg/mL15pg/mL7pg/mL70pg/mL15pg/mL30pg/mL7pg/mL12pg/mL4pg/mL25pg/mL40pg/mL0.
15ng/mL40pg/mL15pg/mL30pg/mL15pg/mL8pg/mL4pg/mL62pg/mL4pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells7.
81~500pg/mL15.
60~1,000pg/mL32~2,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL0.
63~20ng/mL31.
20~2,000pg/mL62.
50~4,000pg/mL156.
25~10000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL62.
50~4,000pg/mL312~20,000pg/mL64~4,000pg/mL62.
50~4000pg/mL3.
125~200ng/mL156.
00~10,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL7.
81~500pg/mL62.
50~4,000pg/mL781.
25~50000pg/mL31.
20~2,000pg/mL31.
25~2,000pg/mL156~10,000pg/mL31.
25~2,000pg/mL125~8,000pg/mL15.
60~1,000pg/mL23.
44~1500pg/mL7.
81~500pg/mL46.
90~3,000pg/mL156~10,000pg/mL0.
312~20ng/mL78~5,000pg/mL31.
25~2000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL12.
50~800pg/mL7.
81~500pg/mL125~8000pg/mL7.
8~500pg/mL31.
25~2,000pg/mLCHE0206CHE0042CHE0118CHE0067CHE0061CHE0179CHE0098CHE0177CHE0105CHE0294CHE0113CHE0047CHE0074CHE0087CHE0122CHE0282CHE0086CHE0209CHE0238CHE0072CHE0073CHE0239CHE0075CHE0277CHE0082CHE0114CHE0033CHE0281CHE0106CHE0092CHE0286CHE0035CHE0244CHE0109CHE0120CHE0108CHE0295CHE0052CHE0027CHE0170CHE0158CHE0300CHE0116CHE0029检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOT1516人细胞因子ELISA试剂盒(HumanCytokineELISAKits)TGF-β3(转化生长因子β3)Thrombomodulin/BDCA-3(凝血酶调节蛋白)Thrombopoietin(促血小板生成素)Thrombospondin-1(人血小板反应蛋白-1)TIM-1/KIM-1(T细胞免疫球蛋白黏蛋白)TIMP-1(基质金属蛋白酶抑制因子1)TIMP-2(基质金属蛋白酶抑制因子2)TL1A/TNFSFS15(肿瘤坏死因子超家族15)TLR2(Toll样受体2)TNF-α(肿瘤坏死因子α)TNF-β(肿瘤坏死因子β)TotalMMP-1(总基质金属蛋白酶1)TRAIL/TNFSF10(肿瘤坏死因子相关凋亡诱导配体)TRANCE/RANKL(肿瘤坏死因子相关激活诱导因子)TSLP(胸腺基质淋巴细胞生成素)Uromodulin(尿调节素)VCAM-1(血管内皮细胞粘附因子1)VEGF(血管内皮生长因子)15pg/mL15pg/mL45pg/mL0.
78ng/mL7pg/mL30pg/mL15pg/mL16pg/mL60pg/mL4pg/mL15pg/mL80pg/mL12pg/mL39pg/mL15pg/mL31pg/mL7pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells31.
20~2,000pg/mL31.
20~2,000pg/mL93~6,000pg/mL1.
563~100ng/mL15.
60~1,000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL31.
25~2000pg/mL125.
00~8,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL156.
00~10,000pg/mL23.
44~1500pg/mL78~5000pg/mL31.
20~2,000pg/mL62.
5~4000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mLCHE0171CHE0149CHE0124CHE0271CHE0132CHE0107CHE0100CHE0288CHE0188CHE0019CHE0260CHE0097CHE0293CHE0297CHE0059CHE0302CHE0110CHE0043检测靶标灵敏度检测范围规格产品编号Adiponectin/Acrp3(脂联素)alpha-Fetoprotein/AFP(甲胎蛋白)BAFF/BLyS/TNFSF13B(B细胞活化因子)BDNF(脑源性神经营养因子)CCL11/Eotaxin-1(趋化因子CCL11)CCL24/Eotaxin/MPIF-2(趋化因子CCL24)CCL5/Rantes(趋化因子CCL5)Chemerin(脂肪因子)ComplementComponentC5a(补体C5a)CRP(C反应蛋白)CX3CL1/Fractalkine(趋化因子cx3cl1)CXCL10/IP10(趋化因子CXCL10)CXCL11/I-TAC(趋化因子CXCL11)CXCL12/SDF-1(趋化因子CXCL12)CXCL15/Lungkine(趋化因子CXCL15)CXCL7/ThymusChemokine-1(趋化因子CXCL7)CXCL9/MIG(趋化因子CXCL9)EGF(表皮生长因子)Erythropoietin(促红细胞生成素)E-Selectin/CD62E(E-选择素)FGFbasic(碱性成纤维生长因子)FGF-21(成纤维生长因子21)G-CSF(粒细胞集落刺激因子)GDF-15(生长分化因子15)GM-CSF(粒细胞巨噬细胞集落刺激因子)GranzymeB(粒酶B)HGF(肝细胞生长因子)IFN-beta(小鼠β干扰素)IFN-γ(γ干扰素)IgA(免疫球蛋白A)IgE(免疫球蛋白E)IGF-I(胰岛素样生长因子-1)IgGtotal(总免疫球蛋白G)IgM(免疫球蛋白M)IL-10(白细胞介素10)IL-12/23(P40)(白细胞介素12)IL-12p70(白细胞介素12p70)IL-13(白细胞介素13)IL-15(白细胞介素15)IL-17A(白细胞介素17A)IL-17F(白细胞介素17F)IL-1ra/IL-1F3(白细胞介素1受体a)IL-1(白细胞介素1)IL-1α(白细胞介素1α)15pg/mL31pg/mL30pg/mL7pg/mL4pg/mL7pg/mL15pg/mL8pg/mL7pmol/l15pg/mL0.
2ng/mL30pg/mL31pg/mL25pg/mL60pg/mL4pg/mL7pg/mL2pg/mL32pg/mL31pg/mL30pg/mL7pg/mL8pg/mL15pg/mL16pg/mL20pg/mL80pg/mL6pg/mL4pg/mL0.
20ng/mL156pg/mL15pg/mL0.
8ng/mL0.
20ng/mL7pg/mL4pg/mL7pg/mL4pg/mL60pg/mL4pg/mL15pg/mL80pg/mL7pg/mL1pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells31.
20~2,000pg/mL62.
50~4,000pg/mL62.
50~4000pg/mL15.
625~1000pg/mL7.
81~500pg/mL15.
60~1,000pg/mL31.
20~2000pg/mL15.
625~1000pg/mL15.
60~1000pmol/l23.
40~1,500pg/mL0.
39~25ng/mL62.
50~4000pg/mL62.
50~4,000pg/mL46.
90~3,000pg/mL125.
00~8,000pg/mL7.
81~500pg/mL15.
60~1000pg/mL3.
91~250pg/mL62.
5~4000pg/mL62.
50~4,000pg/mL64~4000pg/mL15.
60~1000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL40~5000pg/mL156~10000pg/mL12.
5~800pg/mL7.
8~500pg/mL0.
39~25ng/mL312~20,000pg/mL31.
20~2000pg/mL1.
56~100ng/mL0.
39~25ng/mL15.
6~1000pg/mL7.
8~500pg/mL15.
6~1000pg/mL7.
8~500pg/mL125~8000pg/mL7.
8~500pg/mL23.
40~1,500pg/mL156.
00~10,000pg/mL31.
25~2000pg/mL1.
8~125pg/mLCME0103CME0110CME0055CME0120CME0079CME0070CME0048CME0119CME0066CME0104CME0125CME0051CME0073CME0065CME0076CME0075CME0050CME0038CME0126CME0095CME0039CME0040CME0030CME0042CME0026CME0057CME0052CME0116CME0003CME0058CME0029CME0053CME0059CME0060CME0016CME0024CME0013CME0009CME0010CME0041CME0089CME0090CME0015CME0019v检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOT小鼠细胞因子ELISA试剂盒(MouseCytokineELISAKits)HOTHOTHOTHOTHOTHOTHOT17184pg/mL16pg/mL2pg/mL4pg/mL16pg/mL16pg/mL16pg/mL7pg/mL7pg/mL2pg/mL15pg/mL7pg/mL7pg/mL60pg/mL7pg/mL30pg/mL7pg/mL0.
8pg/mL7pg/mL7pg/mL60pg/mL40pg/mL62.
5pg/mL6pg/mL4pg/mL60pg/mL7pg/mL156pg/mL60pg/mL15pg/mL50pg/mL32pg/mL7pg/mL4pg/mL15pg/mL4pg/mL60pg/mL4pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells15.
6~1000pg/mL31.
25~2000pg/mL3.
9~250pg/mL15.
6~1000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL31.
25~2000pg/mL15.
6~1000pg/mL7.
8~500pg/mL31.
20~2,000pg/mL15.
6~1000pg/mL15.
625~1000pg/mL125~8000pg/mL15.
625~1000pg/mL62.
5~4000pg/mL15.
60~1,000pg/mL1.
56~100pg/mL15.
60~1,000pg/mL15.
6~1000pg/mL125.
00~8,000pg/mL78.
10~5,000pg/mL125~16,000pg/mL12.
5~800pg/mL7.
8~500pg/mL125.
00~8,000pg/mL15.
60~1000pg/mL312.
5~20000pg/mL125~8000pg/mL31.
25~2000pg/mL93.
80~6,000pg/mL62.
5~4000pg/mL15.
625~1000pg/mL7.
8~500pg/mL62.
50~4000pg/mL7.
8~500pg/mL125~8000pg/mL15.
6~1000pg/mLCME0001CME0021CME0033CME0028CME0025CME0035CME0036CME0005CME0023CME0006CME0031CME0008CME0002CME0061CME0123CME0046CME0084CME0047CME0078CME0018CME0098CME0099CME0115CME0121CME0062CME0105CME0045CME0124CME0064CME0020CME0032CME0127CME0122CME0004CME0054CME0044CME0043CME0014检测靶标灵敏度检测范围规格产品编号CRP(C反应蛋白)EGF(表皮生长因子)GM-CSF(粒细胞巨噬细胞集落刺激因子)ICAM-1/CD54(细胞间黏连分子1)IFN-γ(γ干扰素)IGF-I(胰岛素样生长因子)IgGtotal(总免疫球蛋白G)IgM(免疫球蛋白M)IL-10(白细胞介素10)IL-17A(白细胞介素17A)IL-1(白细胞介素1)IL-1α(白细胞介素1α)IL-2(白细胞介素2)IL-4(白细胞介素4)IL-6(白细胞介素6)MCP-1(单核细胞趋化蛋白-1)MIP-2(巨噬细胞炎性蛋白2)NGAL/Lipocalin-2(中性粒细胞明胶酶相关脂质运载蛋白)TGF-β1(转化生长因子β1)β-NGF(大鼠神经生长因子-β)TIMP-1(金属蛋白酶1组织抑制剂)TNF-α(肿瘤坏死因子α)TotalMMP-9(总基质金属蛋白酶9)VEGF(血管内皮生长因子)80pg/mL7pg/mL7pg/mL31pg/mL15pg/mL16pg/mL0.
8ng/mL0.
16ng/mL15pg/mL7pg/mL30pg/mL7pg/mL7pg/mL7pg/mL60pg/mL7pg/mL7pg/mL40pg/mL31pg/mL7pg/mL15pg/mL7pg/mL40pg/mL7pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells156.
00~10,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL31.
25~2000pg/mL1.
60~100ng/mL0.
312~20ng/mL31.
25~2,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL15.
60~1,000pg/mL125~8,000pg/mL15.
625~1000pg/mL15.
60~1,000pg/mL78.
10~5,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL31.
25~2,000pg/mL78.
10~5,000pg/mL15.
60~1,000pg/mLCRE0025CRE0030CRE0020CRE0031CRE0002CRE0033CRE0016CRE0017CRE0007CRE0021CRE0006CRE0015CRE0001CRE0004CRE0005CRE0034CRE0009CRE0018CRE0012CRE0019CRE0029CRE0003CRE0026CRE0010检测靶标灵敏度检测范围规格产品编号HOTHOTHOTHOTHOTHOTHOT小鼠细胞因子ELISA试剂盒(MouseCytokineELISAKits)HOTHOTIL-2(白细胞介素2)IL-21(白细胞介素21)IL-22(白细胞介素22)IL-23(白细胞介素23)IL-25/IL-17E(白细胞介素25/17E)IL-27(白细胞介素27)IL-33(白细胞介素33)IL-4(白细胞介素4)IL-5(白细胞介素5)IL-6(白细胞介素6)IL-7(白细胞介素7)IL-8/CXCL1/CXCL1(白细胞介素8)IL-9(白细胞介素9)Leptin(瘦素)LIX(脂多糖诱导的cxc趋化因子)MCP-1(单核细胞趋化蛋白1)MCP-2/CCL8(单核细胞趋化蛋白2)MIP-1alpha/CCL3(巨噬细胞炎性蛋白1α)MIP-1beta/CCL4(巨噬细胞炎性蛋白1β)MIP-2/CXCL2(巨噬细胞炎性蛋白2)MMP-3(基质金属蛋白酶3)MMP-9(基质金属蛋白酶9)Myeloperxidase(髓过氧化物酶)NeutrophilElastase/ELA2(中性白细胞弹性蛋白酶)NGAL/Lipocalin-2(中性粒细胞明胶酶相关脂质运载蛋白)PD-1(程序性细胞死亡因子受体1)PlGF-2(胎盘生长因子2)Prolactin(催乳素)sICAM-1(可溶性细胞间粘附分子1)TGF-β1(转化生长因子β1)Thrombomodulin/BDCA-3(凝血酶调节蛋白)Thrombopoietin(促血小板生成素)TL1A/TNFSF15(肿瘤坏死因子超家族15)TNF-α(肿瘤坏死因子α)TRANCE/RANKL(核因子κB受体活化因子配体)TSLP(胸腺基质淋巴细胞生成素)VCAM-1(血管内皮细胞粘附因子1)VEGF(血管内皮生长因子)大鼠细胞因子ELISA试剂盒(RatCytokineELISAKits)HOTHOTHOTHOTHOTHOT7pg/mL7pg/mL40pg/mL30pg/mL7pg/mL15pg/mL60pg/mL30pg/mL15pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells48/96wells15.
60~1,000pg/mL15.
60~1,000pg/mL78~5,000pg/mL62.
50~4,000pg/mL23.
44~1,500pg/mL31.
25~2,000pg/mL125~8,000pg/mL62.
50~4,000pg/mL31.
25~2000pg/mLCSE0004CSE0003CSE0012CSE0013CSE0001CSE0002CSE0006CSE0008CSE0005检测靶标灵敏度检测范围规格产品编号IFN-γ(γ干扰素)IL-10(白细胞介素10)IL-12/IL-23p40(白细胞介素12/白细胞介素23p40)IL-1β(白细胞介素1β)IL-2(白细胞介素2)IL-4(白细胞介素4)IL-6(白细胞介素6)IL-8(白细胞介素8)TNF-α(肿瘤坏死因子α)猪细胞因子ELISA试剂盒(SwineCytokineELISAKits)1920Glucagon(高血糖素)Insulin(胰岛素)PDGF-BB(血小板衍化生长因子-BB)TotalHIF-1alpha(总低氧诱导因子1α)TotalHSP70/HSPA1A(总热休克蛋白70)7pg/mL7pmol/l31pg/mL60pg/mL80pg/mL48/96wells48/96wells48/96wells48/96wells48/96wells31.
25~2,000pg/mL15.
60~1,000pmol/l62.
50~4,000pg/mL125~8,000pg/mL156~10,000pg/mLCSPE0012CSPE0011CSPE0010CSPE0001CSPE0002检测靶标灵敏度检测范围规格产品编号其他标志物ELISA试剂盒(OtherCytokineELISAKits)SFQ012SFQ007SFQ028SFQ011SFQ027SFQ0103SFQ008SFQ005SFQ0102SFQ001SFQ0101SFQ009包被液标准品封板膜封闭液高吸附酶标板酶结合物稀释液浓缩洗涤液(20X)生物素化抗体生物素化抗体稀释液显色剂/单组份超灵敏TMB样本稀释液终止液100ml支张10ml块16ml25ml支/48T16ml6ml16ml12ml产品编号产品名称产品规格ELISA辅助试剂(ELISAAccessoryReagents)定制化服务产品表ADAMTS13(血管性血友病因子裂解蛋白酶)A-FABP(脂肪型脂肪酸结合蛋白)alpha-Defensin1(防御素α1)alpha-Fetoprotein(甲胎蛋白)Angiopoietin-1(血管生成素1)BCMA/TNFRSF17(B细胞成熟蛋白)BMP-10(骨形态发生蛋白10)BMP-11(骨形态发生蛋白11)BMP-4(骨形态发生蛋白4)BMP-5(骨形态发生蛋白5)BMP-9(骨形态发生蛋白9)CCL11/Eotaxin(趋化因子CCL11)CCL14a/HCC-1(趋化因子CCL14a)CCL15/MIP-1delta/LKN-1(趋化因子CCL15)CCL16/HCC-4(趋化因子CCL16)CCL18/PARC(趋化因子CCL18)CCL23/MPIF-1(趋化因子CCL23)CCL27/CTACK(趋化因子CCL27)CCL28(趋化因子CCL28)CNTF(睫状神经营养因子)ComplementFactorD(补体因子D)ComplementFactorH(补体因子H)CX3CL1/Fractalkine(趋化因子CX3CL1)CytochromeC(细胞色素C)E-Cadherin(E-钙黏蛋白)Endoglin/CD105(内皮因子)Endostain(内皮抑素)ErbB2/Her2(总酪氨酸激酶受体)FABP2(脂肪酸结合蛋白2)FGF-4(成纤维生长因子-4)FGF-9(成纤维生长因子9)FGF-BP(成纤维生长因子结合蛋白)HumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHuman96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells0.
78~50ng/mL62.
50~4,000pg/mL0.
50~32ng/mL78.
10~5,000pg/mL156~10,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL7.
80~500pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL15.
60~1,000pg/mL31.
25~2,000pg/mL31.
20~2,000pg/mL0.
206~150ng/mL0.
63~20ng/mL31.
25~2,000pg/mL187.
00~12,000pg/mL125~8,000pg/mL64~4,000pg/mL62.
50~4,000pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL62.
50~4,000pg/mL23.
40~1,500pg/mLCHE0156CHE0245CHE0050CHE0249CHE0168CHE0102CHE0187CHE0186CHE0183CHE0185CHE0079CHE0217CHE0230CHE0223CHE0231CHE0133CHE0228CHE0225CHE0141CHE0040CHE0207CHE0208CHE0139CHE0023CHE0195CHE0138CHE0119CHE0194CHE0111CHE0049CHE0200CHE0235检测靶标反应种属0.
4ng/mL31pg/mL0.
25ng/mL40pg/mL80pg/mL15pg/mL7pg/mL15pg/mL7pg/mL31pg/mL8pg/mL7pg/mL7pg/mL7pg/mL4pg/mL4pg/mL7pg/mL31pg/mL7pg/mL15pg/mL15pg/mL0.
1ng/mL0.
3ng/mL15pg/mL100pg/mL60pg/mL30pg/mL31pg/mL15pg/mL30pg/mL31pg/mL10pg/mL灵敏度检测范围规格产品编号ELISA试剂盒定制服务四正柏生物秉承以客户为中心的原则,最大程度满足客户实验需求,目前推出ELISA试剂盒定制服务,客户提出需检测的特定靶标,四正柏将为您定制您专属的ELISA试剂盒.
目前,以下列表中的靶标均可接受定制服务.
更多定制靶标,请联系四正柏生物.
定制说明:【最低起订量】96wells/【定制周期】5~6周CUSTOMELISAKITS2122HO-1/HMOX1(血红素加氧酶1)HSP27(热休克蛋白27)IgD(免疫球蛋白D)IL-13Ralpha2(白细胞介素13受体α2)IL-16(白细胞介素16)IL-1ra/IL-1F3(白细胞介素1受体α)IL-20(白细胞介素20)IL-36Ra/IL-1F5(白细胞介素36受体a)LeptinR(瘦素受体)L-Selectin/CD62L(L选择素)MCP-4/CCL13(单核细胞趋化蛋白4)Mesothelin(间皮素)MICA(跨膜糖蛋白)MICB(跨膜糖蛋白)MIP-1α/CCL3(巨噬细胞炎性蛋白1α)MIP-1β/CCL4(巨噬细胞炎性蛋白1β)MIP-3β/CCL19(巨噬细胞炎性蛋白3β)MMP-10(基质金属蛋白酶10)MMP-7(基质金属蛋白酶7)NGFR(神经生长因子受体)Notch1(Notch1蛋白)NRG1-β1(神经调节蛋白)NT-4(神经营养因子4)Oncostatin(抑癌素)proBDNF(脑源性神经生长因子前体)Pro-MMP-1(基质金属蛋白酶1前体)Pro-MMP-13(基质金属蛋白酶13前体)SCF(干细胞因子)SerumAlbumin(血清白蛋白)TLR3(Toll样受体3)TNFRI/TNFRSF1A(肿瘤坏死因子受体1)TotalFGFR2alpha(总成纤维细胞生长因子受体2)TotalFGFR3(总成纤维细胞生长因子受体3)TotalFGFR4(总成纤维细胞生长因子受体4)TotalMMP-8(总基质金属蛋白酶8)TotalSurvivin(生存素)Trappin-2(细胞蛋白酶抑制因子)TWEAK/TNFSF12(肿瘤坏死因子相关弱凋亡诱导因子)Ubiquitin+1(泛素+1)u-PlasminogenActivator/Urokinase(纤溶酶原)VEGFR1/Flt-1(血管内皮生长因子受体1)βcatenin(β-连环蛋白)IGFBP-3(胰岛素样生长因子结合蛋白3)Granulysin(颗粒溶素)80pg/mL15pg/mL4ng/mL0.
2ng/mL7pg/mL20pg/mL30pg/mL50pg/mL0.
15ng/mL40pg/mL4pg/mL35pg/mL31pg/mL70pg/mL4pg/mL7pg/mL7pg/mL15pg/mL15pg/mL31pg/mL40pg/mL60pg/mL15pg/mL15pg/mL40pg/mL30pg/mL31pg/mL15pg/mL1.
5ng/mL50pg/mL7pg/mL80pg/mL130pg/mL80pg/mL31pg/mL31pg/mL15pg/mL30pg/mL60pg/mL30pg/mL70pg/mL150pg/mL62pg/mL7pg/mL96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells156.
00~10,000pg/mL31.
20~2,000pg/mL7.
80~1,000ng/mL0.
312~20ng/mL15.
6~1,000pg/mL39.
10~2,500pg/mL62.
50~4,000pg/mL93.
80~6,000pg/mL0.
312~20ng/mL78.
10~5,000pg/mL7.
81~500pg/mL78~5,000pg/mL62.
50~4,000pg/mL156.
00~10,000pg/mL7.
80~500pg/mL15.
60~1,000pg/mL15.
60~1000pg/mL31.
20~2,000pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL78.
10~5,000pg/mL125~4,000pg/mL31.
20~2,000pg/mL31.
20~2,000pg/mL78.
10~5,000pg/mL46.
90~3,000pg/mL62.
50~4,000pg/mL31.
25~2,000pg/mL2.
50~160ng/mL93.
80~6,000pg/mL12.
50~800pg/mL156.
00~10,000pg/mL250.
00~16,000pg/mL156.
00~10,000pg/mL62.
50~4,000pg/mL62.
50~4,000pg/mL31.
20~2000pg/mL62.
50~4,000pg/mL125.
00~8,000pg/mL64~4,001pg/mL156.
00~10,000pg/mL312.
00~20,000pg/mL125~8000pg/mL15.
6~1000pg/mLCHE0190CHE0191CHE0094CHE0205CHE0014CHE0142CHE0020CHE0173CHE0237CHE0243CHE0221CHE0126CHE0146CHE0147CHE0051CHE0125CHE0203CHE0180CHE0178CHE0163CHE0247CHE0164CHE0039CHE0169CHE0137CHE0197CHE0181CHE0080CHE0157CHE0176CHE0159CHE0236CHE0234CHE0233CHE0099CHE0165CHE0167CHE0077CHE0248CHE0123CHE0152CHE0241CHE0280CHE0284检测靶标灵敏度HumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHumanHuman反应种属检测范围规格产品编号2324定制化服务产品表定制化服务产品表COMP(软骨寡聚基质蛋白)Pentraxin3/TSG-14(穿透素3/肿瘤坏死因子诱导基因14)LysylOxidaseHomolog2(赖氨酰氧化酶样蛋白2)ADAMTS4(金属肽酶含血小板反应蛋白基元4)BMP-9(骨形态发生蛋白9)CCL1/TCA-3(趋化因子CCL1)CCL17/TARC(趋化因子CCL17)CCL21/6Ckine(趋化因子CCL21)CCL22/MDC(趋化因子CCL22)CCL25/TECK(趋化因子CCL25)CCL27/CTACK(趋化因子CCL27)CCL28(趋化因子CCL28)CCL6/C10(趋化因子CCL6)CD40Ligand/TNFSF5(CD40配体)CXCL13/BLC/BCA-1(趋化因子CXCL13)CXCL16(趋化因子CXCL16)CXCL4/PF4(趋化因子CXCL4)FasLigand/TNFSF6(Fas配体)Fas/TNFRSF6/CD95(凋亡调节表面抗原Fas)FGFacidic(酸性成纤维生长因子)Gremlin(细胞因子Gremlin)IFN-zeta/Limitin(ζ干扰素)IL-11(白细胞介素11)IL-15/IL-15RComplex(白细胞介素15及其受体复合物)IL-17A/FHeterodimer(白细胞介素17A/F二聚体)IL-19(白细胞介素19)IL-20(白细胞介素20)IL-24(白细胞介素24)IL-3(白细胞介素3)IL-38(白细胞介素38)LeptinR(瘦素受体)L-Selectin/CD62L(L-选择素)MCP-5/CCL12(单核细胞趋化蛋白5)M-CSF(巨噬细胞集落刺激因子)MIP-1gamma/CCL9(巨噬细胞炎性蛋白1γ)MIP-3alpha/CCL20(巨噬细胞炎性蛋白3α)MIP-3beta/CCL19(巨噬细胞炎性蛋白3β)Pro-MMP-9(基质金属蛋白酶9前体)P-Selectin/CD62P(P-选择素)Resistin(抵抗素)SCF(干细胞因子)VitaminDBP(维生素D结合蛋白)IFN-α(干扰素-α)LIF(白血病抑制因子)20pg/mL110pg/mL0.
2ng/Ml7pg/mL7pg/mL4pg/mL15pg/mL7pg/mL4pg/mL15pg/mL31pg/mL7pg/mL7pg/mL50pg/mL7pg/mL7pg/mL15pg/mL7pg/mL31pg/mL7pg/mL40pg/mL7pg/mL60pg/mL4pg/mL25pg/mL30pg/mL16pg/mL4pg/mL4pg/mL125pg/mL15pg/mL60pg/mL15pg/mL7pg/mL4pg/mL15pg/mL7pg/mL0.
20ng/mL31pg/mL7pg/mL31pg/mL250pg/mL7pg/mL3.
15pg/mL96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells39.
1~2500pg/mL218.
0~14000pg/mL0.
4~24ng/mL15.
6~1000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL7.
8~500pg/mL31.
20~2,000pg/mL62.
50~4,000pg/mL15.
6~1000pg/mL15.
60~1,000pg/mL93.
80~6,000pg/mL15.
60~1,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL62.
50~4,000pg/mL12.
50~800pg/mL93.
80~6,000pg/mL15.
60~1,000pg/mL125~8000pg/mL7.
8~500pg/mL46.
90~3,000pg/mL62.
5~4,000pg/mL31.
20~2000pg/mL7.
8~500pg/mL7.
81~500pg/mL250~16000pg/mL31.
20~2,000pg/mL125.
00~8,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL7.
81~500pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL0.
312~20ng/mL62.
50~4,000pg/mL15.
60~1,000pg/mL62.
50~4000pg/mL469.
00~15,000pg/mL15.
60~1,000pg/mL6.
30~400pg/mLCHE0291CHE0298CHE0299CHE0304CME0111CME0083CME0068CME0118CME0063CME0087CME0086CME0049CME0088CME0102CME0072CME0074CME0071CME0092CME0093CME0097CME0109CME0106CME0007CME0011CME0091CME0012CME0017CME0034CME0027CME0037CME0107CME0096CME0085CME0108CME0082CME0081CME0080CME0100CME0094CME0077CME0056CME0101CME0117CME0128检测靶标灵敏度HumanHumanHumanHumanMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouseMouse反应种属检测范围规格产品编号2526定制化服务产品表Adiponectin/Acrp30(脂联素)CCL20/MIP-3alpha(趋化因子CCL20)CINC-1/CXCL1(细胞因子诱导的中性粒细胞趋化因子1)CNTF(睫状神经营养因子)CXCL3/CINC-2(趋化因子CXCL3)IgA(免疫球蛋白A)L-Selectin/CD62L(L-选择素)TIM-1/KIM-1/HAVCR(T细胞免疫球蛋白粘蛋白1)CRP(C反应蛋白)IL-1α/IL-1F1(白细胞介素1α)Phospho-Akt(S473)(磷酸化Akt(S473))Phospho-ERK1(T202/T204)(磷酸化ERK1)Phospho-ERK1(T202/T204)(磷酸化ERK1)Phospho-ERK2(T185/Y187)(磷酸化ERK2)TotalHIF-2alpha/EPAS1(总低氧诱导因子2α)80pg/mL15pg/mL7pg/mL15pg/mL15pg/mL8ng/mL31pg/mL7pg/mL40pg/mL50pg/mL80pg/mL60pg/mL235pg/mL235pg/mL60pg/mL96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells96/2*96Wells156~10,000pg/mL31.
20~2,000pg/mL15.
60~1,000pg/mL31.
20~2,000pg/mL31.
20~2,000pg/mL16~1,000ng/mL62.
50~4,000pg/mL15.
60~1,000pg/mL78.
10~5,000pg/mL93.
80~6,000pg/mL156~10,000pg/mL125~8,000pg/mL469~30,000pg/mL469~30,000pg/mL125~8,000pg/mLCRE0027CRE0023CRE0013CRE0022CRE0024CRE0014CRE0032CRE0028CSE0007CSE0009CSPE0006CSPE0007CSPE0003CSPE0003CSPE0004灵敏度RatRatRatRatRatRatRatRatSwineSwineOtherOtherOtherOtherHuman/Rat反应种属检测范围规格产品编号检测靶标ELISA试剂盒售前售后服务TECHNICALSUPPORT【ELISA售前咨询】若有任何四正柏ELISA产品技术咨询,请电邮至tech@4abio.
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【提供结果】提供正式实验报告,含标准曲线图.
【服务周期】5~7天,取决于是否需做预实验、样本及待测指标的数量.
【样本要求】人和动物的血清、血浆及细胞培养上清,-20℃保存,避免反复冻融.
引用产品:MouseTNF-αELISAKit作者单位:武汉大学化学与分子科学学院期刊影响因子:21.
95【01】Chu-XinLi,YuZhang,XueDong,etc.
ArtificiallyReprogrammedMacrophagesasTumor‐TropicImmunosuppression‐ResistantBiologicstoRealizeTherapeuticsProductionandImmuneActivation.
AdvancedMaterialsFirstpublished:25February2019.
引用产品:InsulinELISAkits作者单位:武汉大学化学与分子科学学院期刊影响因子:21.
95【02】Di-WeiZheng,LuXu,Chu‐XinLi,etc.
Photo‐PoweredArtificialOrganellesforATPGenerationandLife‐Sustainment.
AdvancedMaterialsFirstpublished:31October2018.
(IF:21.
95).
【03】LinGuo,LihuiLin,DuanqingPei,etc.
ResolvingCellFateDecisionsduringSomaticCellReprogrammingbySingle-CellRNA-Seq.
MolecularCellVolume73,Issue4,21February2019,Pages815-829.
e7.
引用产品:MouseIFN-γELISAKit作者单位:中国科学院广州生物医学与健康研究所再生生物学重点实验室期刊影响因子:14.
248【04】DingkaSong,JunchenMeng,JieCheng,etc.
Pseudo-monasaeruginosaquorum-sensingmetaboliteinduceshostimmunecelldeaththroughcellsurfacelipiddomaindissolution.
NatureMicrobiologyNov15,2018,BSR20181501.
引用产品:HumanIL-8ELISAKit作者单位:清华大学生命科学中心基础医学系免疫学研究所期刊影响因子:14.
174【05】Jin-YueZeng,Mei-ZhenZou,MingkangZhang,etc.
π-ExtendedBenzoporphyrin-BasedMetal–OrganicFrameworkforInhibitionofTumorMetastasis.
ACSNano2018,12(5),pp4630–4640.
引用产品:MouseTNF-αandIFN-γELISAkit作者单位:武汉大学化学与分子科学学院期刊影响因子:13.
942【06】Rong-HuiDeng,Mei-ZhenZou,DiweiZheng,etc.
NanoparticlesfromCuttlefishInkInhibitTumorGrowthbySynergizingImmunotherapyandPhotothermalTherapy.
ACSNano2019PublicationDate:June27,2019.
引用产品:MouseTNF-α,IL-6,IL-12p40,IFN-γELISAKits作者单位:武汉大学化学与分子科学学院期刊影响因子:13.
903【07】WenbiaoWang,GengLi,DeWu,etc.
ZikavirusinfectioninduceshostinflammatoryresponsesbyfacilitatingNLRP3inflammasomeassemblyandinterleukin-1βsecre-tion.
NatureCommunications9,Articlenumber:106(2018).
引用产品:MouseIL-1βELISAKit作者单位:武汉大学病毒学国家重点实验室和生命科学学院期刊影响因子:12.
124【08】FionaMoghaddas,PingZeng,YuxiaZhang,etc.
AutoinflammatorymutationinNLRC4revealsaleucine-richrepeat(LRR)–LRRoligomerizationinterface.
JournalofAllergyandClinicalImmunologyVolume142,Issue6,December2018,Pages1956-1967.
e6.
引用产品:HumanIL-1βandIL-18ELISAKits作者单位:澳大利亚帕克维尔沃尔特和伊莱莎霍尔医学研究所期刊影响因子:13.
258SCI文献引用列表(部分)PUBLICATIONSLIST(PART)2728【09】FanGao,ChiZhang,Wen‐XiuQiu,etc.
PD‐1BlockadeforImprovingtheAntitumorEfficiencyofPolymer–DoxorubicinNanoprodrug.
SmallFirstpublished:20August2018.
引用产品:MouseIFN-γandTNF-αELISAkits作者单位:武汉大学化学与分子科学学院期刊影响因子:9.
598【10】FengZhang,QiangZhang,XiangyangLi,etc.
Mussel-inspireddopamine-CuIIcoatingsforsustainedinsitugenerationofnitricoxideforpreventionofstentthrombosisandrestenosis.
BiomaterialsVolume194,February2019,Pages117-129.
(IF:8.
806).
引用产品:HumanSerpinE1/PAI-1andvWF-A2ELISAKits作者单位:西南交通大学材料科学与工程学院教育部材料先进技术重点实验室期刊影响因子:8.
806【11】Tong-FeiLi,KeLi,QuanZhang,etc.
Dendriticcell-mediateddeliveryofdoxorubicin-polyglycerol-nanodiamondcompositeselicitsenhancedanti-cancerimmuneresponseinglioblastoma.
Biomate-rialsVolume181,October2018,Pages35-52.
引用产品:HumanIL-12p40,MouseIFN-γandIL-2ELISAkits作者单位:武汉大学医学部期刊影响因子:8.
8【15】E.
Wang,X.
Liu,W.
Tu,etc.
Benzo(a)pyreneFacilitatesDermatophagoidesGroup1(Derf1)‐InducedEpithelialCytokineReleasethroughArylHydrocarbonReceptorinAsthma.
AllergyFirstpublished:14April2019.
引用产品:MouseIL-4,IL-13,IFNγ,IL-17A,TSLP,IL-33,andIL-25ELISAKits作者单位:深圳大学附属罗湖医院期刊影响因子:6.
23【16】XiaochengZhou,TinglinYan,ChunmingHuang,etc.
Melanomacell-secretedexosomalmiR-155-5pinduceproangio-genicswitchofcancer-associatedfibroblastsviaSOCS1/-JAK2/STAT3signalingpathway.
JournalofExperimental&ClinicalCancerResearch201837:242.
引用产品:MouseVEGF,FGF-basic,andMMP9ELISAkits作者单位:武汉大学口腔医学院期刊影响因子:6.
217【12】Mei‐ZhenZou,Wen‐LongLiu,Chu‐XinLi,etc.
AMultifunctionalBiomimeticNanoplatformforRelievingHypoxiatoEnhanceChemotherapyandInhibitthePD‐1/PD‐L1Axis.
Small2018,1801120.
引用产品:MouseTNF-α,IL-6,IL-12,IFN-rELISAKit作者单位:武汉大学化学与分子科学学院期刊影响因子:8.
643【13】Wen-XiuQiu,Ming-KangZhang,Li-HanLiu.
Aself-deliverymembranesystemforenhancedanti-tumortherapy.
BiomaterialsVolume161,April2018,Pages81-94.
引用产品:MouseTNF-α,IFN-γ,IL-1β,IL-12p70ELISAKit作者单位:武汉大学生物医学高分子教育部重点实验室期刊影响因子:8.
402【17】FanGao,QianCheng,Miao-DengLiu,etc.
LocalTregulatorycellsdepletionbyanintegratednanodrugsystemforefficientchem-immunotherapyoftumor.
ScienceChina-chemis-tryPublishedJun27,2019.
引用产品:MouseTNF-αandIL-10ELISAKits作者单位:武汉大学化学与分子科学学院期刊影响因子:6.
085【18】DiWu,ChuanTang,YanfangLiu,etc.
Structuralelucida-tionandimmunomodulatoryactivityofaβ-D-glucanpreparedbyfreeze-thawingfromHericiumerinaceus.
CarbohydratePolymersVolume222,15October2019,114996.
引用产品:MouseTNF-α,IL-1βandIL-6ELISAKits作者单位:上海农业科学院食用菌研究所期刊影响因子:6.
044【19】WuSun,XiaojunWang,JialuLi,etc.
MicroRNA-181apromotesangiogenesisincolorectalcancerbytargetingSRCIN1topromotetheSRC/VEGFsignalingpathway.
CellDeath&Disease9,Articlenumber:438(2018).
引用产品:HumanVEGFELISAkit作者单位:天津医科大学肿瘤研究所期刊影响因子:5.
965【20】ChaoJiang,PingXie,RuxuSun,etc.
c-Jun-mediatedmicroRNA-302d-3pinducesRPEdedifferentiationbytargetingp21Waf1/Cip1.
CellDeath&Disease9,Articlenumber:451(2018).
(IF:5.
965).
引用产品:HumanVEGF-AELISAkit作者单位:南京医科大学附属第一医院眼科期刊影响因子:5.
965【21】NingboXia,TaifangZhou,XiaohanLiang,etc.
ALactateFermentationMutantofToxoplasmaStimulatesProtectiveImmunityAgainstAcuteandChronicToxoplasmosis.
FrontiersinImmunology2018;9:1814.
引用产品:MouseIL12p70,INF-γ,IL-10,andTNF-αELISAKits作者单位:华中农业大学农业微生物学国家重点实验室期刊影响因子:5.
511【22】FangX.
,ZhangT.
,YangM.
,etc.
HighCirculatingAlarinLevelsAreAssociatedwithPresenceofMetabolicSyndrome.
CellularPhysiologyandBiochemistry2018;51:2041–2051.
引用产品:HumanTNF-αELISAKit作者单位:重庆医科大学附属第二医院内分泌科期刊影响因子:5.
5【23】QiuyingZhao,QiaozhiWang,JiutaiWang,etc.
Maternalimmuneactivation-inducedPPARγ-dependentdysfunctionofmicrogliaassociatedwithneurogenicimpairmentandaberrantpostnatalbehaviorsinoffspring.
NeurobiologyofDiseaseVolume125,May2019,Pages1-13.
引用产品:HumanIL-6andTNF-αELISAKits作者单位:中国电子科技大学信息生物学中心生命科学与技术学院期刊影响因子:5.
227【24】Wen-diLuo,Jia-weiMin,Wen-XianHuang,etc.
VitexinreducesepilepsyafterhypoxicischemiaintheneonatalbrainviainhibitionofNKCC1.
JournalofNeuroinflammation201815:186.
引用产品:RatIL-1β,IL-6,andTNFELISAKit作者单位:武汉大学基础医学院期刊影响因子:5.
193【25】Xing-LiangYang,XinWang,LinShao,etc.
TRPV1mediatesastrocyteactivationandinterleukin-1βreleaseinducedbyhypoxicischemia(HI).
JournalofNeuroinflammation201916:114.
引用产品:MouseIL-1βELISAKit作者单位:武汉大学基础医学院湖北省发育源性疾病重点实验室生理学教研室期刊影响因子:5.
193【26】Rui-ChengYang,Xin-YiQu,Si-YuXiao,etc.
MeningiticEscherichiacoli-inducedupregulationofPDGF-BandICAM-1aggravatesblood-brainbarrierdisruptionandneuroinflammatoryresponse.
JournalofNeuroinflammation201916:101.
引用产品:MousePDGF-BBELISAKit作者单位:华中农业大学可持续猪生产合作创新中心期刊影响因子:5.
193【14】Xiao-YanHe,Bo-YaLiu,ChangXu,etc.
Amulti-function-almacrophageandtumortargetinggenedeliverysystemfortheregulationofmacrophagepolarityandreversalofcancerimmunoresistance.
Nanoscale2018,10,15578-15587.
引用产品:MouseIL-12p40,IL-6,IL-10,IL-4,TNF-αELISAKits作者单位:武汉大学化学系生物医学聚合物教育部重点实验室期刊影响因子:7.
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