Reist1fatcow

THECHANGESOFMETABOLICPROFILEANDITSASSOCIATIONWITHFATCOWSYNDROMEINHIGHYIELDINGCOWSDURINGDRYPERIODANDAFTERMutluSevinc,VeysiAslanSelukUniversity,FacultyofVet.
Med.
Thisinvestigationwascarriedouton17BrownSwisscattleintheCentralResearchInstituteofKonyaduringthewinterandlastinguntiltheendofspring.
Bloodsamplesweretakenfromthejugu-larveinduringthe7thand8thmonthofgestationandafterbirthandonthe15thdayafterpartu-ration.
BloodgasanalysesandotherchemicalparametersweremeasuredbyusingaBloodGasAn-alyzerandAutoanalyzerInstruments.
Atthesametime,liverbiopsysampleswereobtainedfromeachcowduringtheexperimentinordertoobserveanyfatinltrationtotheliver.
Blood-ionizedcalcium(ICa)andnormalizedcalcium(normCa)concentrationsdecreasedsigni-cantlyatparturation.
Thesameparameetersweresignicantlyelevatedonthe15thdayafterpartu-ration.
Theothermetabolicprolesthatweremeasuredchangedinsignicantlyduringtheexperi-ment.
Fatinltrationtotheliverwasobservedinafewcowsbuttheleveloffatinltrationwasnotsevere.
CONCENTRATEFEEDINGSTRATEGYINLACTATINGDAIRYCOWS:METABOLICANDENDOCRINECHANGESWITHEMPHASISONLEPTINM.
Reist1,2,D.
Erdin1,D.
vonEuw1,K.
Tschümperlin1,C.
Delavaud2,Y.
Chilliard2,H.
Hammon3,C.
Morel3,Ch.
Philipona3,Y.
Zbinden3,N.
Künzi1andJ.
W.
Blum31Inst.
ofAnimalScience,GroupofAnimalBreeding,SwissFederalInst.
ofTechnology,CH-8092Zurich,Switzerland,2HerbivoreResearchUnit,NationalInstituteforAgriculturalResearch(INRA),F-63122St-Genès-Champanelle,France,and3Div.
ofAnimalNutritionandPhysiology,Inst.
ofAnimalGenetics,NutritionandHousing,Univ.
ofBerne,CH-3012Berne,Switzerland.
Relationshipsbetweenleptinandmetabolic,enzymatic,endocrineandzootechnicaltraitswereas-sessedin90multiparousHolsteincows[mean9'500kgenergy-correctedmilk/305d],heldinare-searchfarmfromwk2antepartum(a.
p.
)towk20postpartum(p.
p.
).
Concentrates(8MJNEL/kgDM)werefedbyautomaticfeedersfor24hat30%(C30)or50%(C50)oftotaldrymatter(DM)intake(DMI)fromwk1-10p.
p.
andwerethencontinuouslyloweredto2.
4%and4%untilwk34,resp.
.
Roughage(6MJNEL/kgDM)wasfedadlibitum.
DMIwasmeasuredcontinuouslyduring24h.
Milkyieldandbodyweight(BW)weredeterminedtwice/d.
Milkcompositionwasdetermined4times/wkandmilkacetone(AC)wasmeasuredweekly.
Bloodsamplesforthedeterminationofmetabolites,hormones,enzymesandelectrolyteswereobtainedinwk2a.
p.
andweeklyfromwk1-16andinwk20p.
p.
between0730and0900.
Bodyconditionscores(BCS)andultrasonicmeasure-mentsofbackfatthickness,long.
dorsimusclediameterandfatdepthinthepelvicareawereevalu-ateda.
p.
andinwk1,4,8,12,16and20p.
p.
.
Inwk4p.
p.
,metabolicandendocrineproleswere204Abstracts–Oralpresentationsat11thICPDActavet.
scand.
Suppl.
98-2003studiedin10cowsofC30andC50,resp.
,byhourlybloodsamplingfrom0730-1530.
Metabolitesandenzymesweremeasuredphotometricallyandhormonesweredeterminedbyspecies-specicra-dioimmunoassays.
Results:Roughageintakewashigher,buttotalDMIwasconsiderablylowerinC30thanC50.
Peakmilkproduction(42.
1and43.
8kg/dinC30andC50,resp.
)wasreachedinwk4p.
p.
,butmeanyieldsofC30cowswereonly1.
28kg/dlowerthanofC50cows.
Energybalance(EB)waspositivea.
p.
,reachedanadiratwk2p.
p.
(-46.
0and-29.
6MJNELinC30andC50,resp.
)andthenincreaseduptowk20p.
p.
.
MetabolicstresswasmoremarkedinC30thanC50cows,ex-pressedbylowerlevelsofglucose,leptin,insulin,IGF-1,T3,milkproteinandlactose,buthigherlev-elsofNEFA,BHBA,GHandmilkAC,andafasterdecreaseofBCSandbackfatthicknessinC30thanC50cowsovertherst20wkp.
p.
(P17mmol/l,glucose>8,8mmol/l)isequivalenttoGPX-activities>500U/mgHb.
InthesecasestheSODactivityisonlyslightlydecreased.
TheSODactivitiesdecreasespecialafterrepositionofrightsideDA.
212Abstracts–Oralpresentationsat11thICPDActavet.
scand.
Suppl.
98-2003